Abstract
Virus is the most constraint of rice production in West Africa. Genetic diversity of rice yellow mottle virus (RYMV) was assessed in two rice growing areas of Mali. Forty-three (43) infected rice leaf samples were collected from five locations within the Niger Office and Selingue Development Rural Office rice’s growing areas. The infected samples were confirmed by Reverse Transcriptase Polymerase Chain Reaction (RT-PCR) using primers pair Prymv1 and Prymv2. A total of twenty-six RYMV isolates were obtained and classified into two genotypes A and B with Prymv1 and one genotype with Prymv2. Sequencing of the cDNA fragments with Prymv2 shared isolates into three distinct groups I, II and III. Most of the isolates were under Group I whereas isolates from the Selingue Development Rural Office area and Moussa-Were (Niger Office) area constituted respectively Groups II and III. This study provided baseline data for monitoring the genetic diversity of rice yellow mottle virus in the two rice-growing areas of Mali which could contribute to strategies development for the disease control. Key words: Genetic diversity, reverse transcriptase polymerase chain reaction (RT-PCR), rice yellow mottle virus (RYMV), sequencing, Mali.
Highlights
Yellow mottle disease of rice is caused by a virus that belongs to the group sobemovirus (Hull and Fargette, 2005) and is endemic to only the African continent (IRD, 2014)
Variability was observed among the forty-three (43) samples with 60% of rice yellow mottle virus (RYMV) infected rice samples identified by both primers
Profile A was formed from a band of 300 bp and profile B from three bands of 300, 500 and 1100 pb (Figure 1). These two profiles corresponded to two (2) isolates of rice yellow mottle virus which were identified at Niger Office area whilst isolate A was identified only at Selingue Development Rural Office area
Summary
Yellow mottle disease of rice is caused by a virus that belongs to the group sobemovirus (Hull and Fargette, 2005) and is endemic to only the African continent (IRD, 2014). This disease is more severe in irrigated ricegrowing areas located in sub-Sahara Africa and the most damaging disease in rice cultivation (APCAM, 2004; IRD, 2014; Sere et al, 2013). It was first recorded on IET 2911 and BG 90-2 varieties in 1991 at Kogoni Agricultural.
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