Abstract
Mycobacterium avium subspecies paratuberculosis (Map), is the etiological agent of paratuberculosis, a disease that affects cattle and causes economic losses to the animal husbandry industry. Its opportune diagnosis, in herds, is part of the control measures of the disease; therefore, the objective of this work was to compare paratuberculosis detection of infected bovines with the Fluorescence Polarization Assay (FPA) and Enzyme-linked Immunosorbent Assay (ELISA). Six-hundred and three sera and feces samples, from bovines older than 2 years old were studied. The sera were assessed with the FPA technique using as antigen a protein fraction of 35 kDa obtained from the raw extract of the Map strain 3065, and for ELISA the protoplasmic antigen of the same strain was used. DNA was obtained from the feces and assessed by nested PCR. The correlation of results was established by Kappa Test. The FPA test had sensitivity (Se) of 88.50% and specificity (Sp) of 91.42% (p ≤0.000); for ELISA Se 83.86% and Sp 89.87% (p ≤0.000) were obtained. Concordance (K) between tests was 0.6742%, and when compared with nested polymerase chain reaction (PCR), the FPA test had K = 0.7314%, while for ELISA it was 0.5771%. The FPA technique using as antigen the protein fraction of 35 kDa showed a higher sensitivity and specificity, moreover it was a simple technique for the determination of the antigen-antibody interaction, and therefore it becomes an alternative diagnostic tool to detect paratuberculosis infected bovines. Key words: Paratuberculosis, diagnosis, fluorescence polarization assay (FPA), enzime-linked immunoabsorbent assay (ELISA).  
Highlights
Paratuberculosis or Johne's disease is an infectious contagious disease that is characterized by the production of chronic granulomatous enteritis
The results obtained with the Fluorescence Polarization Assay (FPA) (142/603) and Enzyme-linked Immunosorbent Assay (ELISA) (141/603) tests were similar; the FPA test identified only one more serum positive (Table 2)
Concordance between tests showed a good correlation of FPA when compared to ELISA
Summary
Paratuberculosis or Johne's disease is an infectious contagious disease that is characterized by the production of chronic granulomatous enteritis. Paratuberculosis (Map) (Arsenault et al, 2014). This serious economically important disease has a worldwide distribution with prevalence rate variation from five to 30% (Mortier et al, 2015). Affected bovines suffer chronic enteritis, the signs of which are diarrhea, submandibular edema, weight loss, and low body condition that leads eventually to death (Cirone et al, 2007; Speer et al, 2006). Appetite and general behavior of the animal remain normal during the early stages, but milk production and body condition worsen due to nutrient malabsorption. There is lethargy, depression, bristly hair, hypoproteinemia and submandibular edema (Mortier et al, 2015)
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