Abstract
DnaJ (Hsp40), a heat shock protein, is a molecular chaperones responsive to various environmental stress. To analyze the protective role of DnaJ, we obtained sense transgenic Arabidopsis plants that constitutively expressed elevated levels of DnaJ. In this study, sense transgenic plants show large thinner, fade color and malformed leaves, as well as less floss of back leaves. Plants with enhanced levels of DnaJ in their transgenic sense lines exhibited tolerance to NaCl stress. Under 120 mM NaCl, root length was higher in transgenic sense plants than wild-type plants. In vitro expression system, DnaJ protein shows tolerance to high NaCl. These results suggest that over-expression of DnaJ can confer NaCl-stress tolerance.
Highlights
Heat shock proteins are synthesized by all the cells in response to the various type of environmental stress
DnaJ protein levels of the Arabidopsis leaves were significantly higher than that of other organs (Figure 2).The results showed that expression of DnaJ was higher in the leaves and lower in the roots
Heat shock proteins had been testified to play important roles in helping cells to cope with environmental stress
Summary
Heat shock proteins are synthesized by all the cells in response to the various type of environmental stress. It is well documented that under stressful conditions like infection, heat, NaCl, ABA, etc, heat shock proteins are induced to help cells to tide over these assaults. Heat shock proteins are molecular chaperones involved in a variety of cellular processes including protein folding, protein transport across membranes, regulation of protein degradation, modulation of protein activity and prevention of irreversible protein aggregation (Sun et al, 2001; Lee et al, 1995; Mohd et al, 2006). Plant BiPs, a member of the HSP70 protein family, were shown to be induced by a variety of environmental stresses, including water stress (Cascardo et al, 2000). Over-expression of BiP- enhanced water stress tolerance in transgenic tobacco plants, where Over-expression of tobacco NtHsp 70-1 contributes to drought-stress tolerance in plants (Eun and choo, 2006)
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