Abstract
The banana (Musa acuminata Colla) is considered as an important crop plant due to its high economic value as good dietary source. Here, we analyze the genetic relationship of four different banana varieties that are cultivated in south India. Random amplified polymorphic DNAs (RAPDs) fingerprinting of these banana varieties (Grand Naine, Red Banana, Nendran and Rasthali) carried out by three primers (OPA-19, OPB-18, OPD-16) led to DNA amplification. 43.47% of the amplification products were monomorphic (common to all the genotypes), whereas 30.43% were unique, but only 26.08% revealed the relationship between these genotypes. Key words: DNA fingerprinting, RAPD analysis, Taq DNA polymerase, primers.
Highlights
Traditional methods for testing genetic variability in fruit crops are based on morphological or timeconsuming physiological assays (Scheliro et al, 2001)
Banana is one of the important fruit and vegetable crops of India that is known as universal fruit crop of India
Among the selected primers OPB-18 (Figure 1) produced maximum number of polymorphic 4 bands followed by OPA-19 and OPD-16
Summary
Traditional methods for testing genetic variability in fruit crops are based on morphological or timeconsuming physiological assays (Scheliro et al, 2001). The presently exist biochemical and molecular techniques are more advance to improve these fruit crops. Random amplified polymorphic DNAs (RAPDs) have been used reliably as molecular markers in cultivar characterization for Malus species L. RAPDs have the advantage that the material is processed by an efficient and inexpensive technique without requiring prior knowledge of the genome (Bhat and Jarret, 1995).
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