Abstract
Sugarcane (Saccharum officinarum L) var. 668 was propagated through tissue culture technique: callogenesis at MS media supplemented with different concentrations of growth regulators. Explants were disinfected with 70% ethanol which was sprayed directly on outer most sheathe which covered the tender stem to be used as source of explants. After 15 days, those explants which survived turned into green leaves and were used for the aforementioned study. Different concentrations of growth regulators were applied. For callogensis response from the cultivated explant, combination of Murashige and Skoog (MS) + 2,4-dichlorophenoxy acetic acid (2,4.D) + benzylaminopurine (BAP) with different concentration was applied and were found best for the establishment of callus from shoot culture. Key words: Saccharum officinarum L, callogenesis, growth regulators, explants, shoot culture.
Highlights
Plant tissue culture techniques have become a powerful tool for studying and solving basic and applied problems in plant biotechnology
All 48 explants were grown on the Murashige and Skoog (MS) medium supplemented with different concentrations and combination of auxin and cytokine: MS + 2, 4dichlorophenoxy acetic acid (2, 4-D) (2 mg/L); MS + 2, 4-D (3 mg/L); MS + 2, 4-D 2 mg/L+ benzylaminopurine (BAP) (1 mg/L) and MS + 2, 4-D 3 mg/L + BAP 1 mg/L
All the facilities and equipments were provided by NIFA and Sarhad University of Science and Information Technology Peshawar (SUIT)
Summary
Plant tissue culture techniques have become a powerful tool for studying and solving basic and applied problems in plant biotechnology. As a result of which, plant regeneration through tissue culture technique would be a viable alternative for improving the quality and productivity in sugarcane. On tissue culture of sugarcane from different countries but the first attempts to regenerate plants through in vitro technique were made on sugarcane by Naz (2003). Standardization of protocols for in vitro multiplication of sugarcane through callus culture, axillary bud and shoot tip culture have been reported by many authors (Devi and Srinivasan, 2006).
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