Abstract
Papaver orientale is an important medicinal plant and source of the valuable alkaloids. In this study, callus production and regeneration of P. orientale under in vitro conditions was investigated with different levels of kinetin (Kin), 6-benzylamine purine (BA) and α-naphthalene acetic acid (NAA) on Murashige and Skoog (MS) and Gamborg's B5 (B5) media. For callogenesis, explants as cotyledons-hypocotylswere placed on the solidified MS and B5 media with hormones under 16-h photoperiod and 20°C conditions. Results showed that the best media for callus induction of P. orientale consisted of the MS basic media supplemented with 0.5 mg/l BA and 0.5 or 1 mg/l NAA. Shoots were regenerated in cultures grown on MS medium containing 0.5 mg/l Kin and (0.5 or 1.0 mg/l) NAA and were induced to root on B5 medium containing 1 mg/l Kin and 1.0 mg/l NAA. Key word: Callus induction, in vitro regeneration, Papaveraceae, Papaver orientale.
Highlights
Papaveraceae family have many applications including inhibition of acetylcholinesterase (Cahlikova et al, 2010), antibacterial agent (Bhattacharjee et al, 2010) or inhibition of algae and cyanobacteria in water management (Jancula et al, 2007, 2010)
This study showed that the best medium for callus induction in P. orientale was Murashige and Skoog (MS) supplemented with 0.5 mg/l benzylamine purine (BA) and 0.5 or 1.0 mg/l naphthalene acetic acid (NAA) with an average of 97.7 and 100% explants producing callus (Table 1)
Increasing BA concentration from 0.5 to 1 mg/l caused a reduction of callus production and this reduction was greater at 1 mg/l concentration of NAA
Summary
Callus production and regeneration of the medicinal plant Papaver orientale Rasool Asghari Zakaria*, Maryam Haghighat Hour and Naser Zare. Papaver orientale is an important medicinal plant and source of the valuable alkaloids. Callus production and regeneration of P. orientale under in vitro conditions was investigated with different levels of kinetin (Kin), 6-benzylamine purine (BA) and -naphthalene acetic acid (NAA) on Murashige and Skoog (MS) and Gamborg's B5 (B5) media. Explants as cotyledonshypocotyls were placed on the solidified MS and B5 media with hormones under 16-h photoperiod and 20°C conditions. Results showed that the best media for callus induction of P. orientale consisted of the MS basic media supplemented with 0.5 mg/l BA and 0.5 or 1 mg/l NAA. Key word: Callus induction, in vitro regeneration, Papaveraceae, Papaver orientale
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