Abstract
The present study was performed to detect the molecular and the phylogenetic identification of species that belonging to the genus of Moniezia Blanchard, 1891 which affected intestines of sheep in Al-Diwaniyah city, Iraq; fifty intestine samples were sought for the infestation of Moniezia spp. from the city slaughterhouse from 1 October to 30 November 2017, this tapeworm was found to infest the intestines of 13 sheep. For morphological identify the genus of this tapeworm, eggs from one gravid proglottid of the thirteen worms were examined, polymerase chain reaction (PCR) and the PCR-product-based sequencing were applied on 4 Moniezia tapeworms targeting a specific region of the 18S rRNA gene. The sequencing has shown 2 species of Moniezia, SP1 and SP2 ,these two species revealed close matching on the phylogenetic tree to an according to the current study findings, Moniezia spp. affect on sheep in the city of Al-Diwaniyah, Iraq, these findings give interesting information about the evolution history of this worm in the studied city.
Highlights
The genus of Moniezia are considered as high prevalent worms that infest sheep intestines, the disease conditions by these worms lead to risky-economic crises around the world (Soulsby, 1982; Mazyad and El-Nemr, 2002)
To identify the genus of this tapeworm morphologically, eggs from one gravid proglottid of the thirteen worms were examined (Rahif, 1998); sequencing of the polymerase chain reaction (PCR) products were applied on four Moniezia tapeworms targeting a specific region of the 18S rRNA gene (743bp).The protocol of gSYAN DNA Extraction Kit (Gene aid, USA) was followed to extract the genomic DNA from the mature proglottids of the worms
Fifty intestines were examined for the infestation of Moniezia spp. in the city slaughterhouse; this tapeworm was found to infest the intestines of 13 sheep
Summary
The genus of Moniezia are considered as high prevalent worms that infest sheep intestines, the disease conditions by these worms lead to risky-economic crises around the world (Soulsby, 1982; Mazyad and El-Nemr, 2002). Cyclophyllidea and Anoplocephalidae are the order and the family of this genus respectively, each proglottid has repeated sexual parts for better differentiation of these worms; mites are considered the main intermediate hosts for Moniezia species that provide a source of infestation via feeding on grass (Denegri et al, 1998). Young animals appear to be the main targets for the infestation by M. expansa (Wymann, 2008); Molecular identification and phylogenetic-tree several proglottids that have sensory-organ-based anterior-scolexes, neck, and the strobilus are the main parts of this species. To study the evolution history of Moniezia species in the city of Diwaniyah, Iraq, the present study was initiated to evaluate the identity matching or mismatching of the city species with global species that belonging to this genus
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