English

Abstract

Arbuscular mycorrhizal fungi (AMF) are obligatory biotrophs that have a symbiotic evolutionary relationship with about 80% of all terrestrial plant species. The fungus mainly supplies water and nutrients to the plant and receives photoassimilates. The AMF diversity affects both the competition among species and floristic composition of an area. Fluctuations in the population of this group of microorganisms can cause fluctuations in plant populations above ground. In this work, the AMF community profile in areas with different ages of revegetation was evaluated by morphological identification of spores and denaturing gradient gel electrophoresis (DGGE) techniques. We found 12 AMF species and dominance of the species Glomus macrocarpum determined by spore density. Since the richness level observed in each plot was low and there was predominance of one species of AMF, it can be concluded that these areas still present a high degree of disturbance. It was possible to detect complex band profiles by DGGE analyses for the two plant species studied, Visnia latifolia and Cecropia hololeuca. No relationship between AMF diversity and revegetation time was observed in these areas. Key words: Denaturing gradient gel electrophoresis (DGGE), symbiosis, succession, survey.