Abstract
Combretum molle (Combretaceae) is widely used traditional medicinal plant for treatment of different illnesses in most parts of Africa. However, its in vivo toxic effect is not evaluated yet. Thus, the aim of this study was to evaluate cytotoxic effect of C. molle seed extract on biochemical and hematological parameters using mice. Accordingly, effect of seed extract of the plant on hematological and biochemical parameters and on tissues of different organs were determined. Blood sample collected on the next day of the last dose administration was used for further analysis. Organs were collected in formalin for histological study. Result of the study showed that seed extract of C. molle did not cause significant effects on most red blood cells (RBCs) indices. Also, except eosinophil count, all white blood cells (WBCs) indices showed significant reduction (P<0.05) under use of almost all doses of the seed extract. Level of liver enzymes such as glutamic oxaloacetate transaminase (sGOT) and glutamic pyruvic transaminase (sGPT) showed significant increment (P<0.05) in mice treated with different doses of the plant seed extract, while concentration of albumin was significantly reduced (P<0.05). Similarly, significant increament (P<0.05) in levels of kidney function indicators urea and creatinine was observed. At relatively lower dose (125 mg/kg), the crude extract of C. molle seed showed lower effects on haematological, biochemical and immunological parameters. As dose increased, its cytotoxic effect increased. Key words: Combretum molle, hematological, biochemical, histopathology.
Highlights
Combretum molle is one of the herbal plants belonging to the family Combretaceae which includes 20 genera and about 600 species (Miaffo et al, 2015)
Outcomes of hematological parameter showed that, use of higher doses of crude extract C. molle seed for seven days cause significant (P
As the dose increased to 500 mg/kg, platelet count reduced, but not significantly different from the control group. Other hematological parameters such as mean corpuscular volume (MCV), mean corpuscular haemoglobin (MCH), mean corpuscular haemoglobin concentration (MCHC) and RDW-CV were not affected in mice that received the treatment (Table 1)
Summary
Combretum molle is one of the herbal plants belonging to the family Combretaceae which includes 20 genera and about 600 species (Miaffo et al, 2015). Roots of C. molle seem to have a variety of uses against hookworm, stomach pains, snake bite, leprosy, fever,dysentery, general body swellings, and abortion as well as for swelling of the abdomen, sterility and constipation (Fyhrquist et al, 2012). This plant bark and root in vitro tested in different African countries for treatment of bacteria (Sahlu, 2013; Regassa and Araya, 2012). C. molle bark extract was tested for its in vitro antimicrobial activities (Regassa and Araya, 2012; Amare and Tadesse, 2016)
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