English

Abstract

Nicotine has been reported to induce oxidative stress by producing the Reactive Oxygen Species (ROS). In vitro studies showed that Red grape extract has significant antioxidant activity and can inhibit oxidation. Pathogen free, Wistar strain male albino rats were used in the present study, rats were divided into 4 groups of six rats in each group and treated as follows: Group I. Normal Control (NC) (Control rats received 0.9% saline). Group II. Nicotine treated (Nt) (at a dose of 0.6 mg/ kg body weight by subcutaneous injection for a period of 2 months). Group III. Red grape extract treated (RGEt). (Red grape extract at a doses of 50 mg/ kg body weight via orogastric tube for a period of 2 months). Group IV. Nicotine + Red grape extract treated (Nt+RGEt) (The forth group of rats were received the nicotine + red grape extract as followed by the second and third group). The animals were sacrificed after 24 hours after the last treatment by cervical dislocation and isolated the brain such as the activities of the levels of, Succinate dehydrogenase (SDH), Malate dehydrogenase (MDH), and Isocitrate Dehydrogenase (ICDH) are decreased in nicotine treated rats in the Brain and enhance was observed in the combination (Nt+RGEt), Lactate dehydrogenase (LDH), are increase in nicotine treated rats in the Brain and decreased was observed in the combination (Nt+RGEt), but at 50 mg/kg body weight of Red grape extract found to be more effective. This results stating that Red Grape Extract treated rats are beneficial, especially for the nicotine subjects to improve the brain status. Hence the present study was carried out to evaluate the interaction of nicotine on oxidative enzymes in the brain of male albino rat.