Abstract
Grape seeds (GSs) have been claimed for antidiabetic effects since long. Due to its rich phytochemical potential, current study was aimed to evaluate the antidiabetic effect of GSs powder (GSP) (OPC 95%) on neonatal streptozotocin (nSTZ) induced T2DM rats. STZ (90 mg/kg) was administered intraperitoneally in 48 h old rat pups. After 3 months, 24 T2DM rats were selected by OGTT for 28-days experiment and divided into four groups (n=6) group I: Normal water control [NWC], group II: Diabetic water control [DWC] (10 mL ddH2O/kg bw) group III: Gliclazide treated [GT] (20 mg/kg bw) and group IV: GSP treated group (1.25 g/kg/ bw). Blood were collected by tail cut and cardiac puncture method during the begging and end of the experiment respectively and thereafter serum was separated. Liver was also collected and all samples stored at -20°C freezer until the measurement of fasting serum glucose (FSG), lipid profile, insulin level and liver glycogen content by following standard methods. Statistical analysis was performed considering one-way ANOVA and paired t-test. Oral consumption of GSP significantly (P<0.009) reduced FSG and increased serum insulin (p<0.001) compared with base line value. GT group also ameliorated FSG significantly (p<0.001) compared to DWC group. Moreover, liver glycogen content was also improved by 16% compared with DWC group. Additionally, TG, TC and LDL were significantly reduced (p<0.002, p<0.01, p<0.05 respectively), HDL was increased by 4% through consecutive GSP treatment. Current results suggest that GSP possesses a significant hypoglycemic effect in T2DM rats. Key words: Grape seed powder, T2DM, streptozotocin, glycemic status.
Highlights
Diabetes mellitus (DM) is a chronic metabolic disorder which is characterized by insufficient insulin production from pancreatic beta cell or when the body cannot make proper use of their insulin (Mellitus, 2005)
As well as the fasting serum glucose (FSG) level of other two groups of rats decreased by 6% and 1% in gliclazide treated and normal water control (NWC) treated group, respectively
The findings suggest that Grape seeds (GSs) powder (GSP) (OPC 95%) does not alter normal metabolic parameters like food and water intake
Summary
Diabetes mellitus (DM) is a chronic metabolic disorder which is characterized by insufficient insulin production from pancreatic beta cell or when the body cannot make proper use of their insulin (Mellitus, 2005). In Bangladesh 8.4 million people are affected by diabetes and subjects are prediabetes (that is, IFG & IGT) will be raising 15 million in 2045 (Borgnakke, 2019) This estimation has become a serious health concern for the practitioners and pharmaceutical management globally. In the STZ induced diabetic rat model, grape seeds powder solution decreased the levels of lipid peroxides and carbonylated proteins and enhanced the antioxidant activity (Hogan et al, 2010). In this perspective, the present study was designed to explore the plausible antidiabetic action regarding changes of some biochemical parameters (glucose, insulin and liver glycogen) in nSTZ induced type 2 diabetic model rats by treating grape seeds powder (OPC 95%)
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