Abstract
The nutrient and phytochemical composition of Rosa davurica Pall. fruit (RDF) from China were determined, including sugar, reducing sugar, ascorbic acid, caroteniods and phenolics. RDF was successively extracted with chloroform, ethyl acetate (EA), water-saturated n-butanol, ethanol and distilled water, respectively. Among the five fractions, EA fraction revealed the highest total phenolic content and the strongest antioxidant ability in cyclic voltammograms (CV) and oxygen radical absorbance capacity (ORAC) assays. Moreover, the strongest inhibitory activity against oleic acid-induced (OA-induced) fatty liver in vitro was also the EA fraction. Further, it was separated by a semi-preparative high performance liquid chromatography (HPLC) on C18 column. The most active fraction (EA-FII) of EA in all assays above, which mainly included catechin, quercetin-sedoheptulose and quercetin-94, was identified by LC/MS, HPLC and ultraviolet (UV)/V spectrum analysis. Key words: RDF extracts, composition properties, antioxidant ability, inhibitory activity, oleic acid-induced, HPLC, LC/MS.
Highlights
In today’s modern lifestyle, an increase in dietary fat intake, saturated fat increases the prevalence of obesity and diseases caused by metabolic syndrome, diabetes and steatosis (Damjanovic and Barton, 2008; Cerf, 2007)
Numerous studies suggest that obesity, diabetes, and the metabolic syndrome are closely associated with the disease progression of nonalcoholic fatty liver disease (NAFLD) (Malhi et al, 2006; Utzschneider and Kahn, 2006)
The results reveal that the content of ascorbic acid in wild Rosa davurica Pall. fruit (RDF) (4300.60 mg per 100 g of dry weight) was much higher than the values described in study of Nojavan et al (417.57 mg per 100 g) (2008) and Barros et al (68.04 mg per 100 g) (2010)
Summary
In today’s modern lifestyle, an increase in dietary fat intake, saturated fat increases the prevalence of obesity and diseases caused by metabolic syndrome, diabetes and steatosis (Damjanovic and Barton, 2008; Cerf, 2007). Numerous studies suggest that obesity, diabetes, and the metabolic syndrome are closely associated with the disease progression of nonalcoholic fatty liver disease (NAFLD) (Malhi et al, 2006; Utzschneider and Kahn, 2006). NASH has frequently been encountered in the general population and the biological mechanism of underlying steatosis occurrence and progression to NASH is not entirely understood. In this complex pathogenesis, a twohit hypothesis proposed by Day and James (1998) has been advanced. Natural antioxidant properties in dietary are used as regulatory factor to counteract liver damage
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