Abstract
Shigella sonnei encounter numerous different stresses during their growth, survival and infection. In this study, the effect of stress response to pH and starvation wasinvestigated. We studied the survival, adhesion and the morphology of Shigella after its incubation in several pH. Our results show that after 2 h of incubation, the rate of cell survival was proportional to the decrease of pH of the medium, but we also noted that the reduction of the cells viability is significant for the normal cells compared to starved cells. In addition to that, the results proved the evidence that the pH can influence significantly the hydrophobicity of S. sonnei (normal and starved) and their capacity to produce biofilm on Congo red agar and on polystyrene microplate wells.The atomic force micrographs showed a reduction of the cells size after stress. Key words: Shigella sonnei, starvation, pH, survival, adhesion, morphology.
Highlights
Our results show that after 2 h of incubation, the rate of cell survival was proportional to the decrease of pH of the medium, but we noted that the reduction of the cells viability is significant for the normal cells compared to starved cells
The results proved the evidence that the pH can influence significantly the hydrophobicity of S. sonnei and their capacity to produce biofilm on Congo red agar and on polystyrene microplate wells
Shigella is a member of the Enterobacteriaceae family and causes shigellosis characterized by bacillary dysentery
Summary
Shigella is a member of the Enterobacteriaceae family and causes shigellosis characterized by bacillary dysentery (mucoid bloody stool). Shigella is commonly found in water contaminated with human feces and fecalâoral route is the primary mode of transmission. It is responsible for a worldwide health problem; it is a serious concern in developing countries. It is estimated that Shigella spp. account for 1.1 million deaths and 165 million cases of dysentery annually worldwide. Shigellosis has a high morbidity and mortality rate in children less than 5 years of age. Malnourished children are highly susceptible and Shigella infection further promotes impaired nutrition, recurring infection and retarded growth. Antibiotic resistant strains are continuously emerging treatment regiments become very difficult against shigellosis
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