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Abstract
Recently, zebrafish has shown the potential to become an important in vivo model for diabetes-related research. In this study, we performed a validation study for the establishment of optimal hyperglycemia involving the following factors: alloxan concentrations of 100, 200, 300, and 400 mg/100 ml; exposure times of 30, 60, 120, and 180 min to an alloxan solution and water glucose solution; and water glucose solution concentrations of 1, 2, and 3%. The results have shown that exposure to water glucose solution following alloxan treatment might increase blood glucose level in zebrafish in a dose- and time-dependent manner. The appropriate hyperglycemia zebrafish model was induced in 300 mg/100 ml alloxan solution for 30 min, 1% water glucose solution for 30 min, and water for 1 h. We suggest that our zebrafish model could be an alternative hyperglycemia animal model in the future. Key words: Zebrafish, hyperglycemia, alloxan, blood glucose.
Highlights
Diabetes mellitus (DM) is a type of metabolic disease that is brought about by either insufficient production of insulin or the inability of the body to respond to insulin formed within the system
The blood glucose level of zebrafish exposed to a 100 ml half saline solution of 100 mg alloxan for 30 min was similar to that of the control group
This study reports the preparation of an acute hyperglycemia zebrafish model
Summary
Diabetes mellitus (DM) is a type of metabolic disease that is brought about by either insufficient production of insulin or the inability of the body to respond to insulin formed within the system. The disease can be classified into two different categories: type 1 and 2 diabetes. Type 1 DM is caused by the loss of beta cells found in the islets of Langerhans in the pancreas. The model is induced when either alloxan or streptozotocin is administered to destroy the beta cells, resulting in hyperglycemia (Gohil et al, 2010; Hemalatha et al, 2010; Idan-Feldman et al, 2011; Lee et al, 2011; Liu et al, 2011; Ojezele and Abatan, 2011). Type 2 DM is generally characterized by the body’s resistance to insulin
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