Abstract

Section Nigri is among the most important from the genus Aspergillus, since it possesses economical and biotechnological properties. Its identification is considered quite hard and complex. Due to this fact, Aspergillus taxonomy has been carried out by morphological, biochemical and molecular data. Morphological characterization besides molecular analyses was performed to properly identify Aspergillus section Nigri. A set of 42 Aspergillus section Nigri isolates were obtained from Brazilian Atlantic Rainforest. From these isolates, 16.7% originated from leaf litter layer and 83.3%, from soil. The isolates were divided into three different groups according to analyzed characteristics. 26 were identified as Aspergillus aculeatus, 12 as Aspergillus aculeatinus and 4 belonged to a group called “Aspergillus niger Aggregate”, considered as biseriate. Key words: Fungi, taxonomy, morphological identification, polymerase chain reaction (PCR), ITS.

Highlights

  • Chara acteriza ation of o Aspe ergillu us section Nig gri iso olates from le eaf littter and d soil in n the A

  • Section Nig gri is among the most im mportant from m the genus s Aspergillus s, since it po ossesses economical and biotech hnological prroperties

  • Many authors have been highlighting the importance of polyphasic taxonomy, by combining different identification tools, using morphological, physiological and biochemical characteristics, as well as metabolites production and molecular data. Their main goal is to clarify the complex taxonomy of section Nigri species (Varga et al, 2011; Silva et al, 2011; Simões et al, 2013)

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Summary

Deparrtamento de Entomologia

Rural do Rio d de Janeiro, Brrazil. Departame ento de Microb biologia e Imu unologia Vete erinária, Unive ersidade Fede eral Rural do Rio de Janeiiro, Brazil. Section Nig gri is among the most im mportant from m the genus s Aspergillus s, since it po ossesses economical and biotech hnological prroperties. Its s identificatio on is conside ered quite ha ard and complex. Due to this fact, Aspergillus s taxonomy has been carried outt by morph ological, biochemical a and molecu ular data. Fun ngi from the e genus Asp pergillus hav ve a significa ant imp pact on mod dern society The use of these fung gi as enzymes produ ucers is recog gnized by Foo od and Drug A

Aspergillus ge enus possess
Amplification of ITS region from rDNA by PCR
Leaf litter and soil samples and isolation
Morphological analyses
Amplified fragments sequencing
Similarity and phylogeny analyses
Morphological characterization
Dark Brown to
DNA extraction and rDNA amplification analysis
Sequence similarity analysis of ITS amplified
Conc clusion
Ochratoxigenic Black Aspergilli Isolates from Dried Agricultural
Methods
Full Text
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