Abstract
The cacari ( Myrciaria dubia ) is a native fruit tree from Amazon with high concentrations of vitamin C. This study aimed to adjust a culture medium that meets the nutritional needs for the in vitro development of cacari, evaluating the effect of different concentrations and nutritive culture media, antioxidant, and levels of agar and pH. Three experiments were carried out in a completely randomized design: 1 - woody plant medium (WPM), Murashige and Skoog (MS) and Juan, Antonio, Diva and Silvia medium (JADS) nutritive media with 25, 50, 75 and 100% concentrations; 2 - pH (3.7, 4.7, 5.7 and 6.7) and agar concentrations (0, 3.5, 7.0 and 10.5 g.L -1 ); 3 - Antioxidants [( ascorbic acid (AA), citric acid (CA), polyvinylpyrrolidone (PVP)] and concentrations (0, 100, 200, 300 mg.L -1 ) on the control of phenolic oxidation of stem segments. After collection, the explants were disinfested in a laminar flow chamber, dipping in 70% ethanol for 3 min and 1.5% sodium hypochlorite for 12 min, followed by three washes in distilled and autoclaved water. After disinfestation, the explants were inoculated in 15 × 125 mm test tubes containing 30 mL culture medium, according to each experiment and their respective treatments. After 90 days, the number and length of sprouts (cm) and the oxidation were evaluated. The best results were obtained using the WPM medium at a concentration of 100% with 7 g.L -1 agar, and pH adjusted to 5.7. The use of antioxidants in the tested conditions did not contribute to decrease in oxidation in explants, indicating that there is no need of adding them into the culture medium. Keywords: Camu-camu, in vitro culture, Myrciaria dubia , organogenesis
Highlights
The caçari (Myrciaria dubia (Kunth.) McVaugh) is a native fruit tree from Amazon, widely distributed in the conditions of the Northern Amazon
The use of antioxidants in the tested conditions did not contribute to decrease in oxidation in explants, indicating that there is no need of adding them into the culture medium
Optimum culture medium and concentration for the in vitro regeneration of caçari The analysis of variance showed an interaction between the culture media and concentrations established in this study for the sprout length and oxidation value
Summary
The caçari (Myrciaria dubia (Kunth.) McVaugh) is a native fruit tree from Amazon, widely distributed in the conditions of the Northern Amazon. This species has aroused a great interest of the national and international. Because the species is still in domestication process (Chagas et al, 2012), there is little information about its micropropagation. This technique is important for use in breeding programs of the species, multiplication of high clones with difficult rooting and obtaining plants free from pests and diseases and with high quality. In most cases the culture medium is empirically defined, using as a basis the protocoled culture media, showing no specificity to the species nutritional requirements, the limiting factor of the morphogenetic processes of a given species (Correia, 2006)
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