Abstract
Two expressed sequence EST-SSRs primers were used to show genetic variation and determine a potential link of these markers to salt stress tolerance on two contrasting Medicago truncatula genotypes (Tru 131 tolerant genotype, and Jemalong, sensitive one). The amplification of the DNA were isolated from 10 individual seedlings for each genotype (tolerant and sensitive) with two Expressed Sequence Tag-Simple Sequence Repeat (EST-SSR) primers (MTIC 044) and (MTIC 124) produced a total of 20 amplified products, of which MTIC 124 was polymorphic. The sizes of the alleles detected ranged from 100 to 280 bp. The EST-SSRs markers were polymorphic with an average of 1.33 alleles per primers and gave moderate values of polymorphic information content (PIC) that ranged from 0 to 0.267. The analysis of polymorphism loci for each genotype showed that the tolerant genotype (Tru 131) population had two alleles; genetic diversity index of 0.32 and PIC value of 0.267. The results obtained from unigene database of highly similarity proteins sequences with these loci showed that these two EST- SSRs loci MTIC 044 and MTIC 124 encode GATA transcription factor and cysteine proteinase inhibitor, respectively and were expressed principally in root in M. truncatula. This data suggest that these two loci are involved in salt stress tolerance and the two EST-SSR markers used are appropriate for the studying of salt stress tolerance in M. truncatula. Key words: Medicago truncatula, salt stress, in silico analysis, expressed sequence tag-simple sequence repeat (EST-SSR), UniGene / UniProt databases.
Highlights
Molecular investiga ation off two c contras sting g genoty ypes off Medicago trun ncatulla to sa alt stre ess usiing two exprressed uence tag-sim t mple se equence rep peat
The two loci (EST-SSRs) located on the chromosome 3 (LG3) (Table 1), were chosen from the set of microsatellites developed by Journet et al (2001) in M
Results show that the MTIC 124 locus was more polymorphic (Table 1)
Summary
Molecular investiga ation off two c contras sting g genoty ypes off Medicago trun ncatulla to sa alt stre ess usiing two exprressed uence tag-sim t mple se equence rep peat Adel Am mar Amouri1*, Sripad da M. Fatima a Zohra Fya ad Lamèch he, Driss IIraqi, Fatim ma Henkrarr and Sam mira El Hanafi. ICARDA-INRA Coopera ative Researc ch Project, Intternational Ce enter for Agricultural Rese earch in the D. 14; Accepted 29 September, 201 4 ssed sequen nce EST-SSRs primers were used to show ge enetic variation and dettermine a. Two expres potential link of these e markers to t salt stres ss tolerance e on two c contrasting Medicago trruncatula genotypes (Tru 131 tole erant genoty ype, and Jem malong, sens sitive one).
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