Abstract
The activities of fractions derived from hydroalcoholic extract of Quercus baloot leaves against a dimorphoic pathogenic yeast, Candida albicans (Cl. I. 4043) was evaluated. The hydroalcoholic extract was sequentially fractionated to give n-hexane, dichloromethane, ethylacetate, and n-butanol fractions, which were also subjected to qualitative phytochemical analyses. Preliminary anticandidal screening was carried out using disk diffusion assay with clotrimazole and chloroform serving as positive and negative controls, respectively. Using optimized solvent systems for each fraction, thin layer chromatography was done to separate the secondary metabolites that were evaluated for bioactivity using contact bioautography technique. Minimum inhibitory concentrations of the individual fractions were found using broth microdilution method. Preliminary screening showed all the fractions, apart from aqueous fraction, to have anticandidal potential (zone of inhibition ≥ 10 mm) against test yeast. However, contact bioautography revealed that only dichloromethane fraction was bioactive with inhibition zones at Rf = 0.44 and 0.50, which showed location of inhibitory compound. The MIC of 125 µg/ml also support the presence of anticandidal moieties in this fraction. Phytochemical analyses indicated flavonoids, terpenoids, and steroids in the fractions. Key words: Quercus baloot, Fagaceae, anticandidal activity, contact bioautography.
Highlights
Quercus baloot Griff. belong to oaks family
The activities of fractions derived from hydroalcoholic extract of Quercus baloot leaves against a dimorphoic pathogenic yeast, Candida albicans
Using optimized solvent systems for each fraction, thin layer chromatography was done to separate the secondary metabolites that were evaluated for bioactivity using contact bioautography technique
Summary
The wood of this plant is used as timber, fire-wood and its bark is a source of tannins. Traditional uses of various species of Quercus include utilization in problems of skin and wounds, locomotor organs, and gastrointestinal tract (Viegi et al, 2003). They have been reported for a number of pharmacological activities that include as anti-. Further scientific screenings are required to evaluate the pharmacological potentials of this genus
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