English

Liu Chuanguang,Chen Youding,Li Lijun,Zhou Xinqiao,Chen Dagang,Guo Jie,Li Juchang

doi:10.5897/ajar2018.13826

Abstract

Flag leaf is the most important source of photosynthate for developing rice grains, and flag leaf thickness is an important morphological trait in rice plant-type breeding programs. In the present study, we carried out association mapping for flag leaf thickness in a local rice population which consisted of 86 cultivars derived from breeding programs and planted in large areas in South China. Phenotyping was conducted in the field using nondestructive leaf thickness measurements. Two hundred and thirty-six SSR markers covering 12 chromosomes were employed to genotype the accessions. The association analysis was carried out using a unified mixed-model approach. The Q+K model was selected for investigating marker-trait associations. A total of eleven marker-trait pairs with significant marker-trait associations were identified which were distributed on eight chromosomes. Four of these loci had already been identified as related to flag leaf thickness in previous studies, while the other seven were novel QTLs. The locus PSM163 had the highest r2-marker value of the seven novel loci, explaining 21.54 and 18.49% of the phenotypic variation in 2008 and 2009, respectively. Three of four QTLs, which were detected in a F2 mapping population in the validation study, could correspond to a significant locus in AM, respectively. The six alleles which had the highest phenotypic values at their respective loci should be considered as favored alleles in breeding programs. Pyramiding the favored alleles for flag leaf thickness identified in the study might be a valuable approach to construct an ideal plant architecture in rice breeding. Key words: Rice, flag leaf thickness, breeding programs, association mapping, mixed linear model.

Highlights

Rice is a staple food for about 50% of the global population
The results showed that flag leaf thickness is closely related to the thickness of other leaves on the same stem, suggesting that rice leaf thickness traits share a single genetic system controlled by multiple genes or quantitative trait loci (QTL) (Liu et al, 2014)
Developed in South China from 1949 to 2006, four cultivars introduced from IRRI, and two landraces which were used as core parents in rice breeding programs in South China (Table 1)

Summary

Introduction

Rice is a staple food for about 50% of the global population. With the ongoing reduction in arable landcaused by urbanization and industrialization, breeding rice varieties with greater yield potential will be a very important component of meeting the increased food demand of a growing global population. Genetic improvement of plant morphology is the backbone of increasing rice yielding potential (Khush, 1995; Yuan, 1997). Flag leaf is the most important source of photosynthate for developing rice grains. Over 50% of the carbohydrate accumulated in rice grains is produced by flag leaves (Gladun and Karpov, 1993).

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