Abstract
Young leaf bases and leaf blades about 0.5 cm in height of a carnivorous plant Dionaea muscipula were used as explants for determining the callus multiplication. Explants were cultured on 1/2MS medium supplemented with various concentrations of benzyladenine (BA; 0, 0.1, 0.2, 0.5, 1.0 mg/l). 1/2MS medium supplemented with 1.0 mg/l BA gave the highest average diameter of callus about 0.55 cm after culturing for nine weeks. Callus was subcultured into the same medium every three weeks four times. 1/2MS supplemented with 0.5 mg/l BA gave the highest average plant height, number of leaves, number of roots, and root length. When young shoots about 0.5 cm long treated with a combination of different concentrations (0, 5, 10, 15 and 20 mg/l) of colchicine within different incubation times (24, 48 and 72 h), the survival rate was dependent on the concentration of colchicine and incubation time. Their survival rate was the lowest, when young shoots were soaked in 20 mg/l colchicine for 72 h (70%). Key word: Dionaea muscipula, colchicine, incubation time, micropropagation.
Highlights
Dionaea muscipula Ellis (Venus fly trap) is a carnivorous plant which is endangered and on the brink of extinction, and belongs to the Droseraceae family
Explants of D. muscipula approximately 0.5 - 1 cm in size were cultured on half-strength MS (1/2MS) medium supplemented with various concentrations of BA
The results show that the survival rate of D. muscipula
Summary
Dionaea muscipula Ellis (Venus fly trap) is a carnivorous plant which is endangered and on the brink of extinction, and belongs to the Droseraceae family. This plant is native to the eastern coast of the U.S.A. This ornamental plant is attractive and can be used as a medicinal plant. This plant has been used for years as a source for an anticancer drug and various secondary metabolites which have been used as immunomodulator, antileprosy, antifertility, abortifacient and chitin synthetase inhibitor (Finnie and Staden, 1993; Pakulski and Budzianowski, 1996). There is a number of reports on the in vitro propagation of other carnivorous plants as an effort toward their preservation
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