Abstract
The accidental release of diesel and other hydrocarbon to the environment lead to the pollution of the environment. The search for an environmentally friendly technique for the remediation of such polluted environment is not to be overemphasis. Microorganisms are used in the bioremediation of polluted environments. Hydrocarbon-degrading bacterium isolated from the hydrocarbon exploration site soil of Kukawa North-eastern Nigeria, and was studied for its biodegradation potential of diesel. Culture base techniques using nutrient agar and mineral salt medium supplemented with 1% diesel was used. The bacterium isolate was identified as Enterobacter sp. strain IAA-01. Identification was carried out using 16S rRNA sequencing and molecular phylogeny analysis using the Phylip software. Gravimetric analysis was conducted to measure the percentage biodegradation of the bacterium for 21 days, and confirmed using Gas chromatography (GC) analysis where the bacterium degraded diesel hydrocarbon 78% after 21 days and total degradation was revealed in GC analysis after 21 days. The results prevailed the ability of Enterobacter sp. strain IAA-01 effectively biodegrade diesel oil. Key words: Enterobacter sp., biodegradation, gas chromatography, pollution.
Highlights
The intense increase in production, refining and distribution of crude oil and its products has conveyed with it an ever accumulative problem of environmental pollution (Afuwale and Modi, 2012).The problem of environmental pollution due to oil and oil products is not limited only to oil producing countries but countries with large number of refineries and refining capacity such as Nigeria where the crude oil are being transported and refined, especially through oil producing region are susceptible to oil pollution (Oboh et al, 2006)
Various remediation methods were employed to remediate hydrocarbon polluted soils and water, and the biological method turn to be environment friendly and cost effective, studies have shown that hydrocarbon degrading bacteria are naturally present in these environments and play a very important role in the removal of the pollutants (Allamin et al, 2014)
Bacterial species isolated was screened in order to find out their biodegradation potentials
Summary
The intense increase in production, refining and distribution of crude oil and its products has conveyed with it an ever accumulative problem of environmental pollution (Afuwale and Modi, 2012). The problem of environmental pollution due to oil and oil products is not limited only to oil producing countries but countries with large number of refineries and refining capacity such as Nigeria where the crude oil are being transported and refined, especially through oil producing region are susceptible to oil pollution (Oboh et al, 2006). The regions are characterised by detached, indiscriminate and highly unregulated disposal of petroleum products. Apart from the indiscriminate disposals of oil there are hydrocarbon pollution due to oil spills especially diesels and gasoline in Nigeria. The main objective of this work is to isolate and screen bacteria with the ability to degrade hydrocarbon maximally and subject it to molecular characterization
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