Abstract
Restin, a novel member of the melanoma-associated antigen super family, is cloned from differentiated leukemia cells induced by retinoic acid. Restin may interact with some transcription factors. The present study investigated the effect of restin on activating transcription factor 3 (ATF3), an important protein in numerous physiological situations, using the mammalian two-hybrid system. The results show that ATF3 strongly interacted with restin. To determine the effect of restin on ATF3 activity, an ATF3 reporter plasmid with a luciferase gene was constructed. Hela and NIH3T3 cells transfected with both restin and ATF3 exhibited accelerated ATF3 activity compared with those transfected with only ATF3. This result suggests the role of restin in facilitating ATF3 activity. Restin was also shown to up-regulate ATF3 activity by its interaction with the ATF3 DNA binding domain. Overall, these data suggests the involvement of restin in important cellular modulation processes in Hela and NIH3T3 cells by enhancing ATF3 activity. Key words: Restin, MAGE, ATF3, two-hybrid system, ATF3 reporter, luciferase.
Highlights
The first melanoma-associated antigen (MAGE) encoding gene was isolated from a melanoma cell
The present study investigated the effect of restin on activating transcription factor 3 (ATF3), an important protein in numerous physiological situations, using the mammalian two-hybrid system
Using the mammalian twohybrid system to study the interaction between restin and ATF3 is advisable because of the phosphorylation in ATF3
Summary
The first melanoma-associated antigen (MAGE) encoding gene was isolated from a melanoma cell. Subfamilies (Chomez et al, 2001) They have been categorized into two groups, namely, cancer/testis (CT) and non-CT antigens. MAGE-A, B, C, and E are CT antigens They are expressed in almost all tumors and in normal genital tissues. MAGE-D, F, G, H, and necdin are non-CT antigens and they are mainly expressed in normal tissues including testes, but not in tumor tissues (Francois et al, 2009; Lucas et al, 1998; Reddy et al, 2010). MAGE proteins are closely correlated with neoplasia, wound healing, and reproduction (Aprelikova et al, 2009; Francois et al, 2009; Lucas et al, 1998; Reddy et al, 2010)
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