Abstract
In vitro regeneration of sweet orange (Citrus sinensis (L.) Osbeck Family: Rutaceae) has been performed via direct and indirect organogenesis. For indirect organogenesis, callus was induced and proliferated from leaf explants derived from in vitro grown seedlings on Murashige and Skoog (MS) media containing 2,4-dichlorophenoxyacetic acid (2,4-D) alone or in combination with benzyl adenine (BA) and α-naphthalene acetic acid (NAA). For direct organogenesis, explants were placed on MS media containing BA alone or in combination of NAA and gibberellic acid (GA3). Well-developed microshoots were treated with different concentrations of NAA for rhizogenesis (a two-step procedure). Different responses to these treatments were recorded depending upon the procedure used. It appears that 4.53 μM 2,4-D in combination with 5.37 μM NAA induced 93.33% callus and proliferate 86.67% of callus into 6.93 shoots per explant. Exogenous addition of 4.44 μM BA in combination with 1.54 μM GA3 enhanced shoot multiplication rate significantly (17.73±1.69 shoots/explant) in comparison to control (0.00±0.00 shoots/explant). Microshoots were rooted best (75.00±14.43%) under the treatment 100μM NAA for 48 hrs. and rooted plantlets were transferred to soil, following acclimatization were taken to maturity in the polyhouse. Key words: Malta, Himalaya, benzyl adenine (BA), callus.
Highlights
Citrus fruits are one of the most important commercial fruit crops of the world, cultivated in more than 100 countries (Barlass and Skene, 1999)
Citrus sinensis is a member of family Rutaceae and commonly known as navel orange, sweet orange and „Malta‟ in Uttarakhand, India (Christman, 2003)
The optimal callusing response (93.33%) was observed in Murashige and Skoog (MS) medium supplemented with 2,4-D+naphthalene acetic acid (NAA) (4.53+5.37 μM) followed by 2,4-D+NAA (9.06+5.37 μM) (86.67%)
Summary
Citrus fruits are one of the most important commercial fruit crops of the world, cultivated in more than 100 countries (Barlass and Skene, 1999). Citrus (> 108 million tons), ranks second among the most produced fruit crops of the world after banana (FAO, 2006). Citrus sinensis is a member of family Rutaceae and commonly known as navel orange, sweet orange and „Malta‟ in Uttarakhand, India (Christman, 2003). Due to high „vitamin C‟ content and antioxidant potential of C. sinensis, it is consumed worldwide, considered as one of the major commercial fruit crops of the world (Kiong et al, 2008)
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
