Abstract
The expression of the UDP glucose: flavonoid-3-Oglucosyltransferase (UFGT), which encoded the last enzyme of the anthocyanin pathway, was under developmental control as well as affected by external stimuli such as ABA. Three fragments of the 1.38 kb upstream region of the UFGT gene from Litchi (Litchi chinesis Sonn.) were fused to the GUS-coding region, and the expression of these constructs was analyzed in onions. To characterize the cis-regulatory functions of the promoters for enzymes in anthocyanin biosynthesis, we examined onions carrying a series of nested UFGT promoter-β-glucuronidase (GUS) fusion for GUS activity by histochemical staining. The AE-box and ELI-box in anthocyanin biosynthesis of angiosperm were found in UFGT promoter. MRE which was MYB binding site involved in light responsiveness, control the transcription of genes in anthocyanin biosynthesis. The region of -910 to -344 in UFGT promoter showed high activities in the three parts. Although the expression characteristics were indistinguishable from those of the full-length promoter, we observed differences in UFGT promoter regulation for the different construct. The results suggested that region of 910 to 344 of UFGT promoter had multiple functions in the expression under the various developmental stages and stress conditions in litchi. Key words: Flavonoid-3-Oglucosyltransferase (UFGT), promoter, litchi, transient expression.
Highlights
The litchi (Litchi chinesis Sonn.) is a tropical and subtropical fruit tree native to southern China, Taiwan, Bangladesh and Southeast Asia, and cultivated in many parts of the world
The AE-box, and ELI-box in anthocyanin biosynthesis of angiosperm were found in UDP glucose: flavonoid-3-Oglucosyltransferase (UFGT) promoter
MRE which was MYB binding site involved in light responsiveness, control the transcription of genes in anthocyanin biosynthesis (Grotewold et al, 1994; Jin et al, 2000; Moyano et al, 1996; Sablowski et al, 1994; Sainz et al, 1997; Sugimoto et al, 2000; Tamagnone et al, 1998; Yang, 2001)
Summary
The litchi (Litchi chinesis Sonn.) is a tropical and subtropical fruit tree native to southern China, Taiwan, Bangladesh and Southeast Asia, and cultivated in many parts of the world. The UFGT sequences were identical in white and redskinned sports including the promoter region, suggesting that the phenotypic change from white to red could be the result of a mutation in a regulatory gene controlling the expression of UFGT (Kobayashi et al, 2002). We reported the isolation of the promoter of UFGT gene in the anthocyanin biosynthetic pathway.
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