Abstract
An actinomycete, isolated from soil samples of western Sichuan Plateau of China, was identiï¬ed as Streptomyces alboflavus SC11 by 16S rDNA analysis, and also cultural, morphological and physiological characteristics of the strain were recorded. From the fermentation broth of the strain, an antimicrobial ingredient was isolated and elucidated as desertomycin A, mainly by analysis of electrospray ionization tandem mass spectrometry (ESI-MS/MS), 1H nuclear magnetic resonance (NMR) and 13C NMR spectra data. Desertomycin A exhibited good antimicrobial potential against the pathogenic bacteria and fungi. Minimum inhibition concentration (MIC) values of desertomycin A were 3.9, 7.8 and 7.8 μg/ml against Bacillus cereus, Bacillus subtilis and Staphyloccocus aureus,respectively. Key words: Streptomyces alboflavus SC11,Desertomycin A, 16S rDNA, Antimicrobial activities.
Highlights
Soil microorganisms provide a huge and excellent resource for the isolation and identification of agricultural and medicinal important natural products (Lin and Liu, 2010)
The strain exhibited good growth on Gause’s synthetic agar, starch casein agar (SCA), Czapek’s agar, ISP3, ISP4 and ISP5 while the growth was moderate in glucose-asparagine agar and ISP6, but no growth was observed on ISP2
In this study, based on the results of morphological, physiological characterizations and 16S rDNA gene sequence analysis, strain SC11 was been identified as S. alboflavus SC11, from which one active constituent was obtained and identified as a known compound
Summary
Soil microorganisms provide a huge and excellent resource for the isolation and identification of agricultural and medicinal important natural products (Lin and Liu, 2010). 60% of antibiotics developed and applied for agricultural pur-pose were isolated from the Streptomyces genus (Berdy, 2005; Junker et al, 2009), such as Blatididin S (Takeuchi et al, 1958), polyoxins (Suzuki et al, 1965; Isono et al, 1965), Validamycin (Iwasa et al, 1970, 1971a, 1971b) etc. Those promising progress have emphasized that it is necessary to continue the research on screening of new antibiotics from various regions of soils. A strain numbered SC11, exhibiting strong and stable antimicrobial activity against agricultural pathogenic fungi and bacteria, was selected for further study
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