Abstract
The purpose of this study was to evaluate the performance of the Drigalski agar supplemented with 2 mg/L of ceftazidime (DC) for selective isolation of expanded-spectrum beta-lactamase (ESBL) producing Enterobacteria in clinical samples. The Drigalski agar supplemented with 2 mg/L of ceftazidime was compared with the chromIDTM ESBL medium (bioMerieux, Marcy l'Etoile, France). Six strains from the collection of reference center for antibiotics of the Pasteur Institute of Paris and 247 clinical samples were used to calculate the performance and the intrinsic characteristics of DC medium, respectively. The main ESBL producing Enterobacteria consist of Escherichia coli (n = 31; 44.3%), Klebsiella pneumoniae (n = 19; 27.1%) and Enterobacter cloacae (n = 12; 17.1%). ESBL producers were confirmed by synergy testing. The sensitivity (Se), specificity (Sp), positive predictive value (PPV) and negative predictive value (PPN) after 24 h of incubation at 37°C were respectively 98.6, 98.7, 98.6 and 98.7%. The study shows that DC agar is a sensitive and specific medium for selective isolation of ESBL producing Enterobacteria, but the character chromogenic agar chromIDTM ESBL remains an additional advantage in the identification of strains. Key words: Expanded-spectrum beta-lactamase (ESBL), Enterobacteriaceae, sensitive culture medium, specific culture medium, predictive culture medium.
Highlights
Beta-lactams are the main family of antibiotics that include the largest number of molecules and the most used in the world
The Expanded- Spectrum Beta-Lactamase (ESBL) plasmid associated with fluoroquinolone resistance were studied in Ivory Coast in 2008 and this study showed a prevalence of 27.2% of qnr genes associated with producing extended-spectrum beta-lactamase (Guessennd et al, 2008)
It is used as a differential selective medium for Gram-negative bacilli in general (Enterobacteriaceae and certain nonfermenters such as Pseudomonas) and inhibits Grampositive bacteria
Summary
Beta-lactams are the main family of antibiotics that include the largest number of molecules and the most used in the world. This widespread use is due to their broad spectrum of activity, low toxicity, effectiveness and affordability for certain molecules (Livermore, 1995; Dosso, 2000). International License Number U2A 1446* U2A 2252* U2A 2239* U2A 2240* U2A 1528* U2A 1878* Strain
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