Abstract
Proteomic analysis by 2-dimensional electrophoresis (2-DE) of the longissimus dorsi muscle in finishing pigs (LY × D) grown on a diet supplemented with mugwort powder found roughly 300 spots on a polyacrylamide gel image. Among them, the expression levels of 12 spots were higher than those of the control group. From the results of peptide mass fingerprinting (PMF) analysis, nine proteins were characterized: myosin light chain 2V, elF-5A, myosin light chain 1, F1-ATPase chain D, peroxiredoxin-2, Kelch-related protein 1, SLA-7, glycine amidinotransferase, and Tpi 1. The other three spots with increased expression were identified by chemically assisted fragmentation-matrix-assisted laser desorption/ionization (CAF-MALDI) sequencing as serum albumin precursor and two different types of myoglobin. Taken together, these results indicate that mugwort powder has the potential to improve the quality of pork meat. Key words: 2-DE, longissimus dorsi muscle, meat quality, mugwort, pig, proteome.
Highlights
Because proteins play critical roles in transport, immune responses, storage, and structure, and serve as members of metabolic pathways, they are essential factors for the maintenance of life of all organisms
Among 20 selected growing landrace (LYxD) pigs of body weight 75 ± 4 kg, half were grown for 50 days on the basal diet, whereas the other half was grown on the mugwort powder diet
Longissimus dorsi muscle tissues of the carcass were harvested from four pigs that exhibited good breeding in each group, and these were used for the experiment
Summary
Because proteins play critical roles in transport, immune responses, storage, and structure, and serve as members of metabolic pathways, they are essential factors for the maintenance of life of all organisms. Pigs fed mugwort powder improved their average weight gain, protein content, and the lightness of their meat and fat (Kim et al, 2008). This study used comparative 2-DE to investigate global changes in protein expression in longissimus dorsi muscle associated with dietary mugwort supplementation.
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