Abstract
Chlorogenic acid (CGA) is one of the compounds found in coffee beans and other parts of coffee plant. However, its relative content in different coffee plant parts is least researched. Therefore, this study was designed to determine the contents of CGA in coffee leaves and beans. Green coffee beans and leaves were collected from South part of Ethiopia to study the content of CGA using UV/Vis spectrometer with liquid-liquid extraction method in dichloromethane. Results indicated that mean percentage of CGA in green coffee beans and leaves are in the range of 5.96±0.01 to 6.40±0.02 % and 1.94±0.01 to 2.31±0.01%, respectively. On top of that, the percentage difference of CGA between green coffee beans and leaves was at least about 63%, with beans taking the upper hands. These results showed that there is statistically significant content of CGA in green coffee beans than green coffee leaves. Keywords: CGA, Coffea arabica, coffee leaves, concentration, green coffee beans.  
Highlights
Coffee is one of the most widely consumed beverages throughout the world due to its pleasant taste, aroma, stimulant effect and health benefits (Gebeyehu and Bikila, 2015)
The Chlorogenic acid (CGA) concentrations of green coffee beans were statistically different when considered in all the study areas
We report comparative study on determination of concentration of CGA in green coffee beans and leaves with UV/Vis spectrophotometer though liquid–liquid extraction of dichloromethane solvent
Summary
Coffee is one of the most widely consumed beverages throughout the world due to its pleasant taste, aroma, stimulant effect and health benefits (Gebeyehu and Bikila, 2015). Coffee plant is categorized among the medicinal plants because studies on beans and fleshy organs of the coffee plant including leaves have revealed that these organs are found to have generous amounts of secondary metabolites such as phenolic compounds, esters of hydroxycinamic acids and mangiferin which have high level of antioxidant properties and antiinflammatory effects on humans (Campa et al, 2012). Chlorogenic acid (CGA) is one of the compounds found in coffee beans and other parts of coffee plant. The most abundant are the caffeoylquinic acids (Clifford et al, 2003) They account for approximately 80% of the total chlorogenic acid content (Farah et al, 2005).
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