Abstract
Albizia amara, belonging to the family Fabaceae is a valuable economic, medicinal and multipurpose drought tolerant tree commonly found in dry forests of India. Keeping view of its economic importance, a protocol was developed for rapid clonal multiplication ofA.amara by means of plant tissue culture. In the present study, development of complete plantlets via induction of multiple shoots from seedling derived cotyledonary node explant and their successful rooting and acclimatization is reported. Cotyledonary node explants were excised from 12-15 day old aseptic seedlings to initiate culture for multiple shoot induction on MS medium. Among the different plant growth regulators tested either in single or in combination, BAP 1 mg/l, BAP 1 mg/l + Kn 2 mg/l, BAP 1 mg/l + NAA 1 mg/l were effective in inducing multiple shoots from cotyledonary node explants. Shoot elongation was prominent at Kn 0.25 mg/l concentration. In vitro rooting experiments were successful on half strength MS medium fortified with NAA 1 mg/l. Key words: Albizia amara, tree, micropropagation, axillary bud multiplication.
Highlights
Albizia amara (Roxb.) Boiv., belonging to the family Fabaceae, is a valuable economic, medicinal and multipurpose drought tolerant tree commonly found in dry forests of India
Seeds were subjected to repeated washing for 3 to 4 times with sterilized double distilled water (DDW), the seeds were soaked in sterile DDW and incubated at 25 2°C for 24 h
Cotyledonary nodes cultured on MS medium fortified with BAP 1 mg/l showed highest number of multiple shoots (6.84 0.30 shoots/ explant) with average shoot length of 3.20 0.54 (Table 1)
Summary
Albizia amara (Roxb.) Boiv., belonging to the family Fabaceae, is a valuable economic, medicinal and multipurpose drought tolerant tree commonly found in dry forests of India. The wood of Albizia amara is purplish brown with lighter bands, very hard and strong, used for cabinets in building and agriculture purpose. The bark of the tree yields gum, which is used for ulcers (Kashyapa and Ramesh, 1992) and molluscidal activity (Ayoub and Yankov, 1986). The leaves contain a flavanol glycoside namely 4’O–menthylrutin and they are extensively used as herbal cosmetic for hair maintenance. Budmunchiamines, spermine macrocyclic alkaloid (Mar et al, 1991) extracted from the seeds of A. amara were found to interact with DNA by inhibiting the catalytic activity of DNA polymerase, RNA Polymerase, and HIV reverse transcriptase.
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