Abstract
Since ancient times, traditional medicine has used plant extracts for the treatment of diseases and there has been recent interest in the chemopreventive properties of compounds derived from plants. In the present study, we investigated the antioxidant potentials of extracts of Globularia alypum leaves with the Ferric Reducing/Antioxidant Power (FRAP) and Reducing Power (RP) assays and we evaluated their effects on the genotoxic of nifuroxazide (NF) in the SOS Chromotest. Aqueous, petroleum ether, ethyl acetate and methanol extracts were prepared from powdered G. alypum leaves, and characterized qualitatively for the presence of tannins, flavonoids, coumarins and sterols. Our results showed significant antioxidant capacity by the FRAP and RP methods. Aqueous extract showed the highest antioxidant capacity with a value of 8.9 mM TE 4.5 mM TE by FRAP and the reducing power method respectively. All the extracts significantly decreased the genotoxicity induced by10 µg of NF (_S9).The maximum inhibitory effect towards NF induced genotoxicity by the tested G. alypum extracts was obtained in the presence of Escherichia coli PQ37 strain at a dose of 250 µg/assay of the aqueous extract (99.95%). Our results show that extracts of G. alypum leaves are a significant source of compounds with antigenotoxic and antioxidant activity and thus may be useful for chemoprevention. Key words: Globularia alypum, SOS chromotest; antigenotoxic effect, antioxidative activity.
Highlights
Antioxidants such as glutathione, α-tocopherol, ascorbic acid, superoxide dismutase, catalase, peroxidase, etc. are produced within cells to prevent the deleterious effects of potentially harmful Reactive Oxygen Species (ROS), such as superoxide anion radical (O2-.) hydroxyl radical (.OH) and peroxyl radical (ROO.), are constantly generated in vivo both by aerobic metabolism and exogenous sources such as UV radiation, environmental pollution and the diet
All extracts did not contain coumarins, whereas, a high quantity of sterols was detected in the petroleum ether extract
Our results showed significant antioxidant capacity by the Ferric Reducing/Antioxidant Power (FRAP) and reducing power methods, (Table 2)
Summary
Antioxidants such as glutathione, α-tocopherol, ascorbic acid, superoxide dismutase, catalase, peroxidase, etc. are produced within cells to prevent the deleterious effects of potentially harmful Reactive Oxygen Species (ROS), such as superoxide anion radical (O2-.) hydroxyl radical (.OH) and peroxyl radical (ROO.), are constantly generated in vivo both by aerobic metabolism and exogenous sources such as UV radiation, environmental pollution and the diet. ROS can affect the quality of foods which are rich in polyunsaturated fatty acids, reducing the nutritional content of foods and promoting the development of food rancidity and off-flavours. Their addition to food is an effective way to prevent the development of various undesirable compounds that result from lipid oxidation. Both natural and synthetic antioxidants have been shown to enhance product stability, quality, and shelf life (NourEddine et al, 2003). Biological activities should be discussed in relation to secondary metabolic composition
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