Abstract
This work aimed to develop a protocol for the in vitro establishment, multiplication, rooting and ex vitro acclimatization of Mimosa pudica L., a species used in folk medicine and with pharmacological activity. Aseptic cultures were established from seeds inoculated in MS medium, without growth regulators, followed by an in vitro stabilization phase in culture medium supplemented with 2.22 μM BAP. The cultures were transferred to MS medium supplemented with different cytokinins, combined or not with an auxin, aiming its large-scale propagation. The culture medium supplemented with 5 µM BAP plus 0.5 µM NAA provided the highest multiplication rate and top quality plantlets. The combination of 0.6 µM TDZ plus 0.05 µM NAA resulted in higher multiplication rates than in response to combination of BAP plus NAA, although the subsequent maintenance of the cultures in a medium without growth regulators has resulted in low regenerative response. In vitro rooting of micro-cuttings was high even in the absence of auxins. Over 90% of plantlets transferred to the greenhouse survived after the acclimatization phase. Acclimatized plants presented normal vegetative and reproductive development. The procedures established in the present study allow a massive production of M. pudica plants for further pharmacological studies. Key words: Biodiversity conservation, ex vitro acclimatization, in vitro rooting, micropropagation, Mimosa pudica.
Highlights
Mimosa pudica L., popularly known in Brazil as dormideira, sensitiva, mimosa, and maria-fecha-a-porta, is a perennial and native species of Tropical America, which can be found in Tanzania, South Asia, SouthEast Asia and many Pacific Islands having near-frequent rainfall (Patra et al, 2016)
The culture medium supplemented with 5 μM BAP plus 0.5 μM NAA provided the highest multiplication rate and top quality plantlets
The procedures established in the present study allow a massive production of M. pudica plants for further pharmacological studies
Summary
Mimosa pudica L., popularly known in Brazil as dormideira, sensitiva, mimosa, and maria-fecha-a-porta, is a perennial and native species of Tropical America, which can be found in Tanzania, South Asia, SouthEast Asia and many Pacific Islands having near-frequent rainfall (Patra et al, 2016). The growth is annual and, despite presenting erect stem when young, they become creeper plants at the adult phase. M. pudica is used in folk medicine, mainly in Asian countries, whereas its extracts are attributed diuretic, astringent and antispasmodic activities. In vitro and in vivo analysis of flavonoids and phenolic compounds of leaves, stems, and seeds of the M. pudica have found high antioxidant, antimicrobial, anticancer and antidiabetic activities, justifying its use in different diseases treatments (Doss et al, 2011; Kaur et al, 2011; Zhang et al, 2011; Joseph et al, 2013; Gunawardhana et al, 2015; Tunna et al, 2015; Jose et al, 2016; Muhammad et al, 2016; Patro et al, 2016)
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