Abstract
A bract is a non-inflorescence structure that exists in many plant species. In daylilies, the bract is biologically functional and is the object of the present tissue culture study. After sterilization, bract explants were treated with 6-benzylaminopurine (BA) and thidiazuron (TDZ) that were used individually or in combination in Murashige and Skoog (MS) nutrients medium, under room environmental conditions, to study its capacity to induce shoots in vitro. The results were successful. Both direct and indirect shoot organogenesis were observed. Although variably, all nineteen cultivars that were investigated induced multiple shoots. TDZ was the most potent chemical stimulus for shoot organogenesis. The results also showed no significant correlation between shoot conversion potential and genotype or treatment. Key words: Daylily, tissue culture, plant organogenesis, bract, Hemerocallis, plant regeneration, thidiazuron.
Highlights
Daylilies are a monocot of great socio-economic and research values that thrive primarily because of its floral beauty and landscaping applications (Hansen, 2007; Rodriguez-Enriquez and Grant-Downton, 2013; Cui et al, 2019; Li et al, 2020)
The present report pioneers successful shoot organogenesis in daylily bract tissue, demonstrating that it can be reliably applied as a primary explant for in vitro propagation
Explants cultured on control medium without growth regulators did neither induce organogenesis nor callus and subsequently necrotized (Figure 2A)
Summary
Daylilies are a monocot of great socio-economic and research values that thrive primarily because of its floral beauty and landscaping applications (Hansen, 2007; Rodriguez-Enriquez and Grant-Downton, 2013; Cui et al, 2019; Li et al, 2020). They are increasingly justified for medicinal usage and pharmaceutical studies (Du et al, 2014; Farcas et al, 2019).
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