Abstract
Open reading frame (ORF7) of transmissible gastroenteritis virus (TGEV) plays important functions in regulating virus virulence. In this study, the ORF7s were cloned from non-porcine TGEV isolates (HYM-09, HYM-09-1 and HYM-09-2), respectively. Sequence analyses showed that there existed some new unique mutations in the three non-porcine TGEV ORF7s, and which had relative farther genetic relationship and evolution distance to ORF7s of other TGEV strains. The study can serve as a foundation for further insight into the virulence change or mutation trend of TGEVs. Key words: Non-porcine transmissible gastroenteritis virus (TGEV), open reading frame (ORF7), phylogenetic analysis, homology modeling.
Highlights
Transmissible gastroenteritis (TGE) is an acute highly contagious disease of pig, and the occurrences of TGE have become more sporadic
Sequence analyses indicated that all the open reading frame 7 (ORF7) of HYM09, HYM-09-1 and HYM-09-2 consisted of 237 nucleotides coding for a basic protein of 78 amino acids
We found the pI value of HYM09-1 ORF7 was lower than others, which might be caused by new mutation sites in the HYM-09-1 ORF7
Summary
Transmissible gastroenteritis (TGE) is an acute highly contagious disease of pig, and the occurrences of TGE have become more sporadic. Wild and domestic carnivores (dog, cat, fox and mink) are suggested as potential subclinical carriers of transmissible gastroenteritis virus (TGEV), serving as reservoirs between seasonal epidemics (Saif and Sestak, 2006). The open reading frame 7 (ORF7) of TGEV is a virulence gene that can regulate host cell defenses and extend the period of virus dissemination (Cruz et al, 2011). Since ORF7 is a virulencerelated gene and nucleotide change in ORF7 might influence TGEV virulence, it is necessary to isolate the ORF7 from non-porcine TGEV because these nonporcine hosts are important transmission media of TGEV and may induce changes in virulence of TGEV. The aim of the present study was to generate new information with respect to TGEV strains isolated from non-porcine hosts
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