Abstract
Pinus merkusii is an important species in Vietnam with many economic and biological contributions. The information on diversity within and between populations of a species is necessary for plantation programs, breeding and conservation strategies. Genetic diversity of three Vietnamese populations (NA, QB and QN) was analyzed using the random amplified polymorphic DNA (RAPD) markers. Nine RAPD primers produced 82 markers, 77 of which were polymorphic with 93.9% of polymorphism. The results showed higher genetic variation within populations (72%) than between populations (28%) and low Nei’s genetic differentiation index among populations (0.1867). The populations also clustered based on PCoA analysis where cluster I included NA and QB populations and Cluster II, the QN population. These results suggest that P. merkusii populations in Vietnam is necessary to develop the genetic resources. Keywords: DNA markers, genetic diversity, Pinus merkusii , random amplified polymorphic DNA (RAPD), Vietnam
Highlights
Pinus merkusii is a tropical forest tree grown and planted naturally across the south Equator
These results suggest that P. merkusii populations in Vietnam is necessary to develop the genetic resources
The random amplified polymorphic DNA (RAPD) markers were successful for analyzing genetic variation in Vietnam pine populations
Summary
Pinus merkusii is a tropical forest tree grown and planted naturally across the south Equator This species is distributed naturally and artificially in Southeast Asia including Vietnam, Laos, Cambodia, Thailand, Malaysia, China and the Philippines (Cooling, 1968; Farjon, 2005; Razal et al, 2005; Theilade et al, 2000). It can grow from 30 to 2000 m above the sea level (Cooling, 1968; Santisuk, 1997). This species has been extremely exploited which reduced areas of its habitats, habitat quality which led to dramatic decrease in natural
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