Abstract
Trichoderma viride, the most promising biocontrol agent is able to control a wide range of plant pathogens. It is one of the most widely used species against plant disease and can improve the plant growth and crop yields. Biomass yields and sporulation of this fungus depends on the culture conditions (culture method), nutritional requirements (carbon and nitrogen source, mineral elements, carbon concentration, carbon to nitrogen ratio), together with environmental factors including water potential, pH, dark/light cycle and temperature. The study optimized the best culture conditions for biomass yields of T. viride TV-1: spore suspension on the basal medium (sucrose 19.00 g, soy peptone 4.06 g, K2HPO4 1.00 g, KCl 0.50 g, MgSO4 0.50 g, FeSO4 0.01 g and 17.00 g agar (Bactor)) for the first stage culture of 4 days under room condition for fungal growth, then they were transferred to sporulation medium (cellobiose/yeast extract, with the carbon concentration of 2 g/L and carbon to nitrogen ratio of 10:1, ZnSO4.7H2O 0.25 g/L, Na2MoO4.2H2O 0.125 g/L, H3BO4 0.05 g/L and 17.00 g Bactor) for 4 days, together with the environmental factors combination of water potential -3.9MPa/pH 8/0 h light/23°C for biomass yields, and -3.9 MPa /pH 3/0 h/23 ℃ for sporulation yields. These results provided important information on mass production (including biomass and spore yields) of this potential biocontrol fungus. Key words: Nutrition, environment, biomass, sporulation, Trichoderma viride.
Highlights
Increasing concern for health and environmental hazards associated with the use of chemical pesticides lead to serious consideration of sustainability in agriculture.Biocontrol agents (BCA) are promising potential alternatives to reduce the input of chemical pesticides and residues on postharvest fruits (Costa et al, 2001; Pal and Kumar, 2013)
The first level the study choose was the best for sporulation of T. viride TV-1 by one-factor-at-onetime method, while after the orthogonal matrix method, the study found that certain nutrition was not combined in the first level of environment, which means that the orthogonal method was necessary for optimizing the sporulation culture conditions including nutritional and environment factors
Though other studies concern is mainly on the orthogonal matrix method, which focuses on nutritional components or just environmental factors, while this study combined them together
Summary
Biocontrol agents (BCA) are promising potential alternatives to reduce the input of chemical pesticides and residues on postharvest fruits (Costa et al, 2001; Pal and Kumar, 2013). 1997; Zhao et al, 1998; Ejechi, 2001; Boby and Bagyaraj, 2003; Ji et al, 2005), being the most promising BCA able to control a wide range of plant pathogens (Guo et al., 2002), and can improve plant growth and crop yields (Kolembet et al, 2001). Production of Trichoderma BCA is less prevalent till
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