Abstract
Metallo-elements have strong affinity for sulfhydryl group (-SH) of glutathione (GSH) present in tissues. It is very important and interesting to study the reaction of gallium nitrate and glutathione as biomarker of glutathione role in detoxification and conjugation in whole blood components (plasma and cytosolic fraction). The effect of gallium nitrate different concentrations was examined on GSH present in whole blood components. Decrease in GSH level was dependant on gallium nitrate concentration. The decrease in GSH level of whole blood components was more prominent with the time of incubation of gallium nitrate. Decrease in the concentration of reduced glutathione may be due to the interaction of reduce glutathione and gallium nitrate to form oxidized glutathione (GSSG) or gallium-glutathione complex. This change in GSH metabolic status provides information regarding the role of GSH in detoxification of gallium nitrate. The effect of gallium metal on glutathione in blood components wasdiscussed in this study in in vitro condition as a model for in vivo condition. Key words: Gallium nitrate, reduced glutathione (GSH), whole blood, plasma, cytosolic fraction (CF), oxidized glutathione (GSSG), Di-thiobis-dinitro-benzoic acid (DTNB).
Highlights
Gallium can be effectively used for suppression of bone
This study shows the influence of gallium as gallium nitrate on glutathione level in whole blood constituents, plasma and cytosolic fraction of whole blood in vitro which may accounts for a model for in vivo interaction
Effect of gallium nitrate on the plasma GSH concentration of whole blood Concentration of GSH was determined in each test tube in isolated plasma fraction having different concentrations of gallium nitrate (100 to 1000 μM)
Summary
Its role in the treatment of hypercalcemia of malignancy and Paget’s disease of bone (Bockman and Bosco, 1994; Bockman et al, 1995) is due to its antiresorptive activity (Warrell and Bockman, 1989). GSH is present in two forms: Reduced state (GSH) and oxidized state (GSSG). Glutathione is present in reduced state (GSH) and is more than 95% of the total (GSH+GSSG) content. GSH and GSSG act as a thiol redox-couple and have role in gene regulation and intra-cellular signal transduction (Dalton et al 1999). Glutathione in reduced state (GSH) has important roles in cell division, immune-response, signal processes control, programmed cell-death, some xenobiotics and heavy metals detoxification (Ogawa, 2005; Meister and Anderson, 1983). GSH is very reactive from physico-chemical point of view and conjugate to other molecules including the heavy metal ions because
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