Abstract
Eight microsatellite loci were isolated using an enrichment protocol from Ericerus pelaChavannes, representing the first microsatellite loci available for the white wax scale. All loci were polymorphic within E. pela. Allele numbers range from six to eleven with the observed heterozygosity (HO) ranging from 0.0000 to 1.0000. These primers screened in this study provide a foundation for population genetic studies of the white wax scale. Key words: Microsatellite marker, white wax scale, population genetic.
Highlights
The Chinese white wax scale is a famous resource insect of China, the wax secreted by the scale was widely used in wax candle production, wax printing, engraving print and Chinese medicine in China about one thousand years ago, while it is been used in chemical, pharmaceutical, food and cosmetic industry (Chen and Feng, 2009)
The white wax scale widely distributed in most parts of China, Japanese and Korea peninsula from subtropics region to temperate region and formed different geographic populations adapting to different climate (Long, 2004; Wang and Xu, 2006; Chen and Feng, 2009)
Among the primer pairs tested in this laboratory
Summary
The Chinese white wax scale is a famous resource insect of China, the wax secreted by the scale was widely used in wax candle production, wax printing, engraving print and Chinese medicine in China about one thousand years ago, while it is been used in chemical, pharmaceutical, food and cosmetic industry (Chen and Feng, 2009). The white wax scale widely distributed in most parts of China, Japanese and Korea peninsula from subtropics region (north latitude 24°) to temperate region (north latitude 44°) and formed different geographic populations adapting to different climate (Long, 2004; Wang and Xu, 2006; Chen and Feng, 2009). Such adaptation has a strong impact on the genetic variation among populations (Chen et al, 1998). For population genetic analyses and breeding studies of the white wax scale, it is important to isolate and characterize its microsatellite loci
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