Abstract
The gene differential expression of Phytophthora sojae during early stage (0 to 7 h) of sporangium formation was studied by differential display reverse transcription polymerase chain reaction (DDRT-PCR) in the present study. Thirty out of 78 primer combinations were selected and 90 stable differential bands were obtained. Thirty-nine positive differential fragments were found in the experiments by using the reverse northern blot, in which 36 sequences of differential fragments were obtained after sequencing. Homology search and analysis were tested by BLASTX in the P. sojae genome database. The results showed that 23 fragments had significant similarities with proteins in P. sojaegenome database, 15 of which matched with the known functional proteins involved in metabolism, cellular processes, and signal transduction processes. Data mining and bioinformatics analysis on 4 fragments which redundancy was greater than 5 showed that sequence AR15-258 had a very high expression level in Phytophthora mycelium, which may be related to mycelium infection or hunger; AR10-263 and GR1-304 were only found in Phytophthora, which might be unique expression in this genus. The analysis and research on important regulatory genes controlling sporangium formation of P. sojae was a foundation for studying of key genes controlling growth and development of P. sojae. Key words: Phytophthora sojae, sporangium formation, mRNA differential display, differential fragments.
Highlights
Phytophthora root rot of soybean, caused by infection of Phytophthora sojae, is a worldwide recognized soybean disease due to its large infection area and severity (Tyler, 2007)
The gene differential expression of Phytophthora sojae during early stage (0 to 7 h) of sporangium formation was studied by differential display reverse transcription polymerase chain reaction (DDRTPCR) in the present study
The results showed that 23 fragments had significant similarities with proteins in P. sojae genome database, 15 of which matched with the known functional proteins involved in metabolism, cellular processes, and signal transduction processes
Summary
Phytophthora root rot of soybean, caused by infection of Phytophthora sojae, is a worldwide recognized soybean disease due to its large infection area and severity (Tyler, 2007) It has been listed as one of the destructive diseases of soybean (Tyler et al, 2006). Previous studies indicated that two genes are important in formation of sporangium of Phytophthora infestans, one of which is Pigpb, which codes G-protein β-subunit, Pigpb1-silenced mutants form very few sporangia (Latijnhouwers and Govers, 2003). Another is piCdc, which expresses in sporangiophore initials, coding Cdc proteins to regulate mitosis and the cell cycle (Ah-Fong and Judelson, 2003).
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