Abstract
Barrisal is an herbal preparation of Hamdard Laboratories (Waqf) Pakistan that has been used as hepatoprotective agent. In this study, the protective effects of Barrisal against liver damage were evaluated in carbon tetrachloride (CCl4)-induced chronic hepatotoxicity in rats. Four groups (I, II, III, IV) of Sprague-Dawley (SD) rats were used and Groups I and II were orally fed with saline and Barrisal (10 ml/kg), respectively for 14 consecutive days while Groups III and IV were orally fed with saline, Barrisal (10 ml/kg) and then a single dose of CCl4(0.3 ml/kg body weight in a 20% olive oil) was injected intraperitoneally 30 min after the last dose of Barrisal and the animals were starved for 24 h. The degree of protection was measured using biochemical parameters like serum glutamate oxaloacetate transaminase (SGOT) and serum glutamate pyruvate transaminase (SGPT), alkaline phosphatase (ALP), total protein and albumin. Data obtained from results were compared using student’s t-test (paired and unpaired) and one-way analysis of variance (ANOVA), significant differences were determined (Tukey honestly significant difference (HSD) test) using the Minitab 13 computing program. Values of P<0.05, were considered to be significant. The results showed that the treatment with Barrisal significantly lowered the CCl4-induced serum levels of hepatic enzyme markers (GOT, GPT, ALP, and total bilirubin, total cholesterol, total protein) indicated hepatoprotective effect of the Barrisal. Histopathological examination of liver sections confirmed that, pre-treatment with Barrisal reduced the hepatic damage induced by CCl4. Barrisal reduced the incidence of liver lesions including hepatic cells cloudy swelling, lymphocytes infiltration, hepatic necrosis, and fibrous connective tissue proliferation induced by CCl4 in rats. Therefore, the results of this study suggest that Barrisal could protect liver against CCl4-induced oxidative damage in rats, and this hepatoprotective effect might be because of its antioxidant and free radical scavenger effects. Key words: Barrisal, hepatoprotective, carbon tetrachloride (CCl4), hepatic enzymes marker, liver, kidney.
Highlights
Barrisal syrup prepared by Hamdard Laboratories (Waqf) Pakistan is composed of only Aloe vera syn
This study investigated the protective ability of Barrisal on liver injury induced by carbon tetrachloride (CCl4) in rats
Toxicological and hepatoprotective effects of Barrisal have been studied on four groups of rats and mice as already described in materials and methods
Summary
Barrisal syrup prepared by Hamdard Laboratories (Waqf) Pakistan is composed of only Aloe vera syn. The medicinal plant Aloe vera is used traditionally. It is a xerophytic plant which includes trees (Aloe ferox), shrubs and herbs and herbs (A. barbadensis). It has large fleshly leaves, carrying spines at the margin and resemble to some extent the agave or century plant (Agave Americana Linne) (Capasso et al, 1998). Pharmacological activities reported so far for its gel, polysaccharides, polypeptides, anthrones and chromones include antibacterial (Tian et al, 2003), anti-inflammatory (Hutter et al, 1996), and immunoprotective (Strickland et al, 2004) activities
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