Abstract
The present study characterized and identified pear cultivars growing in the southern region of Minas Gerais State, Brazil, using microsatellite markers. Nineteen (19) pear cultivars were collected from two sites of Southern Minas Gerais State: Ribeirao Vermelho and Lavras. DNA was extracted from newly formed leaves and amplified using 21 simple sequence repeat (SSR) markers (NH001c, NH002b, NH005b, NH007b, NH008b, NH009b, NH011b, NH013b, NH012a, NH014a, NH015a, NH017a, KA4b, KA5, KA14, KA16, KB16, KU10, BGA35, BGT23b and HGA8b). The data was analyzed by examining unweighted pair group method with arithmetic mean (UPGMA) clustering, principal coordinate analysis, the Shannon index, and the expected heterozygosity for each primer. The markers used were efficient in separating the Asian and European cultivars as well as hybrids. The primer KA16 was noteworthy in distinguishing the species Pyrus communis , Pyrus pyrifolia and Pyrus betulaefolia by its different banding patterns. Grouping by principal coordinates was similar to UPGMA clustering, forming two distinct groups among the Asian and European pears. The Shannon index and the expected heterozygosity had medium values of 0.440 and 0.300, respectively. The pear cultivars from Southern Minas Gerais usually show low productivity, mainly due to the lack of improved strains for less-demanding weather (warmer) conditions. The selection of the most suitable materials would contribute considerably to overcoming this problem, and the markers selected here can be used in separating cultivars - and presumably contribute to the selection of better matrices. Key words: Pyrus sp., Rosaceae, microsatellite, heterozygosity.
Highlights
Gerais Statte, Brazil, us sing microsa atellite marke ers
The selection s of tthe most suitable materials would c contribute considerably to overco oming this problem, p and d the marke ers selected here can be e used in se eparating cultivars - and a presuma ably contribu ute to the selection of bettter matrices s
The 21 primers generated 189 fragments; of these, 156 were polymorphic loci and all were used in the analyses
Summary
Gerais Statte, Brazil, us sing microsa atellite marke ers. Nineteen n (19) pear c cultivars werre collected from two sites of So outhern Mina as Gerais Sta ate: Ribeirão o Vermelho and Lavras. MA) clusterin ng, principall coordinate analysis, the Shanno on index, and d the expecte ed heterozyg gosity for eac ch primer. Grou uping by principal coorrdinates was s similar to UPGMA clu ustering, form ming two distinct groups among the Asian and Euro opean pears s. The selection s of tthe most suitable materials would c contribute considerably to overco oming this problem, p and d the marke ers selected here can be e used in se eparating cultivars - and a presuma ably contribu ute to the selection of bettter matrices s. Rossaceae (genu us Pyrus) tha at are native to Europe and a mportant speccies are Pyru us communis s, Asia; the most im Pyruss pyrifolia, Pyyrus serotina a and Pyrus b bretschneiderri
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