Abstract
Elephant foot yam (Amorphophallus paeoniifolius) is a vegetatively propagated stem tuber crop. In this investigation we describe a highly competent and reproducible in vitro propagation of the plant from corm bud, petiole and young leaf explants. Friable callus was initiated from all the explants on modified MS medium (half the concentration of NH4NO3 and KNO3) supplemented with 0.5 mg l-1 each of benzyl amino purine (BAP), α-naphthalene acetic acid (NAA) and 2,4-dichloro phenoxy acetic acid (2,4-D). Shoot regeneration from calli was optimal on modified MS medium supplemented with 5.0 mg l-1 BAP and 1.0 mg l-1 NAA. Microcorms, capable of producing micro shoots all over the surface, were induced from the callus at a frequency of 90% on shoot regeneration medium supplemented with 5% sucrose. Rooting was 100% on modified liquid MS medium augmented with 5.0 mg l-1 Indole 3- butyric acid (IBA). A 100% survival rate of plantlets on transplantation to soil: sand: coir pith mixture was recorded. Key words: Callus, elephant foot yam, in vitro, microcorms, regeneration, somatic embryo.
Highlights
Optim mization of in n vitro regene eration n and m microc corm indu uction in elep phant foot f ya am (Am morph hophalllus paeo oniifoliius)
The method of surface sterilisation followed for corm bud, petiole and leaf explants of elephant foot yam proved to be effective in getting 70% of contamination free cultures
In the present study, modified Murashige and Skoog (MS) medium with half the concentration of NH4NO3 and KNO3 was used instead of basal MS medium due to the prominent occurrence of necrosis and yellowing in the initial stages of development observed in preliminary studies, which inhibited the callus induction and further growth
Summary
Optim mization of in n vitro regene eration n and m microc corm indu uction in elep phant foot f ya am (Am morph hophalllus paeo oniifoliius). An efficient in vitro callus multiplication and regeneration protocol in elephant foot yam was developed using corm bud, petiole and leaf explants. The source plants of elephant foot yam were grown in open field of ICAR-Central Tuber Crops Research Institute (CTCRI), Kerala, India.
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
