Abstract
Research into plant-derived endophytic fungi has grown in recent decades. Endophytic fungi still have enormous potential to inspire and influence modern agriculture. In this study, the endophytic fungi DZY16 isolated from Eucommia ulmoides Oliv. was tested for its bioactive components and antimicrobial activities using phenol-sulfuric acid method, high performance liquid chromatography method and growth inhibition measurements. The results show that variation trend of extracellular polysaccharide content at different growth stages of the strain DZY16 and the maximum content of extracellular polysaccharide was 2.02 g/L at the sixth day.Moreover, the fermentation broth of the DZY16 contained guanosine, uridine and adenosine; the contents were 1.54 mg/g, 1.07 mg/g and 1.36 mg/g respectively. On the other hand, the strongest antimicrobial activity was exhibited by the acetylacetate extract of strain DZY16 against Rhizoctonia solani and Gibberella zeae, showing 59.84 and 70.86% respectively. The strain DZY16 was identified by internal transcribed spacer (ITS) sequence as belonging to Nigrospora. The results indicate that the endophytic fungi DZY16 of the plant E. ulmoides Oliv. is a promising source of novel bioactive compounds. Key words: Eucommia ulmoides Oliv., endophytic fungi, extracellular polysaccharide, nucleotides, antimicrobial activity.
Highlights
Endophytic fungi are microorganisms that reside in living plant tissues, apparently without inflicting negative effects
The content of extracellular polysaccharide of DZY16 was slowly increased at lag phase and logarithmic phase of the strain DZY16, the content of extracellular polysaccharides had a trend of rapid growth at stationary phase of the strain DZY16, and the maximum content of extracellular polysaccharide was 2.02 g/L at the sixth day
Qualitative High-performance liquid chromatography (HPLC) analysis showed that the fermentation broth of the strain DZY16 had uridine, guanosine and adenosine; the type of nucleoside was more than that of the reported fungi Phomopsis sp. from Azadirachta indica A
Summary
Endophytic fungi are microorganisms that reside in living plant tissues, apparently without inflicting negative effects. They are quite ubiquitous and have been found in all plant species examined to date (Arnold et al, 2000). Is one of the most valuable timber resources in China, and the only species both in its genus and in its family (Ma et al, 2007). This natural resource is in short supply because of the overcollection of the wild plant. On the basis of the above studies of endophytic fungi, it is feasible to study the products of
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