Abstract
Water and ethanol extracts (WE and EE) from the dried sample of brown alga (Sargassum boveanum) were prepared and examined for its phenolic compounds. Amount of total phenolic compounds in WE was about 17 ± 0.492 mg catechin equivalent (CE)/g of dry sample, using Folin-Ciocalteu method. The antioxidant activity (AOA) of WE was high at about 90% inhibition of peroxidation of linoleic acid with 7 mg dry sample/ml solvent. The IC50 of the WE sample and catechin which was used as the positive control with the hemoglobin catalyzed linoleic acid peroxidation method, were (mg/ml): 3.82 and 0.0713, respectively. The IC50 of the WE sample in terms of CE was 18.76 mg CE/g dry sample. The WE sample exhibited noticeable scavenging effects in DPPH free radical scavenging assay. The radical scavenging activity (RSA) was about 94% at 3 mg dry sample/ml solvent. The phenolic constituent appears to be responsible at least in part, for the observed AOA of the algal extract. The results suggest that this alga could be a potential source of natural antioxidant. Key words: Antioxidant activity, brown algae, Sargassum boveanum, polyphenols, radical scavenging activity, linoleic acid peroxidation.
Highlights
During aerobic life different types of oxygen derivatives such as superoxide anion, singlet state oxygen and hydroxyl radicals along with peroxides and transition metals, are produced
The antioxidant activity (AOA) tests is categorized into two main groups: assays in which by using a lipid substrate, the level of oxidation inhibition is measured and the other tests group includes assays in which capacity of the test system for expressing free radical scavenging activity (RSA) is determined (Sanchez-Moreno, 2002)
Chemical assay based on the removal of stable DPPH free radical has been shown to indicate the presence of reducing compounds in terms of hydrogen donation capacity in the test system
Summary
During aerobic life different types of oxygen derivatives such as superoxide anion, singlet state oxygen and hydroxyl radicals along with peroxides and transition metals, are produced. The antioxidant activity (AOA) tests is categorized into two main groups: assays in which by using a lipid substrate, the level of oxidation inhibition is measured and the other tests group includes assays in which capacity of the test system for expressing free radical scavenging activity (RSA) is determined (Sanchez-Moreno, 2002).
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
