Abstract
The genus Quercus (Fagaceae) includes the most important woody plants with decidious and evergreen species in Northern hemisiphere. They have a problematic taxonomy because of widespread hybridization between the infrageneric taxa. Turkey is one of the most important region of the world according to oak species number and variation. In this study, species belonging to evergreen oaks in Turkey were investigated to solve taxonomic problems and to design the limit of taxa by using random amplified polymorphic DNA (RAPD) data. Here, three species of evergreen oaks known as Quercus coccifera , Quercus ilex and Quercus aucheri were studied in all area located and made the comparison within and among species studied using ten RAPD markers. As a result; it can be stated that the presence of the three species in Ilex section is clear. Furthermore, existence of two infraspecific taxa or two seperate taxa in species level within Q. coccifera may be quite possibly considered. Key words: Quercus ilex , Quercus coccifera , Quercus aucheri , random amplified polymorphic DNA (RAPD).
Highlights
The genus Quercus is one of the most diversified groups of the trees of temperate zone in north Hemisiphere with more than 500 species (Govaerts and Frodin, 1998; Tovar-Sanchez and Oyama, 2004; Olfat and Pourtahmasi, 2010; Maryam Ardi et al, 2012)
Species belonging to evergreen oaks in Turkey were investigated to solve taxonomic problems and to design the limit of taxa by using random amplified polymorphic DNA (RAPD) data
Three species of evergreen oaks known as Quercus coccifera, Quercus ilex and Quercus aucheri were studied in all area located and made the comparison within and among species studied using ten RAPD markers
Summary
The genus Quercus is one of the most diversified groups of the trees of temperate zone in north Hemisiphere with more than 500 species (Govaerts and Frodin, 1998; Tovar-Sanchez and Oyama, 2004; Olfat and Pourtahmasi, 2010; Maryam Ardi et al, 2012). The species analysed were Quercus coccifera, Quercus aucheri and Quercus ilex known as evergreen oaks. These are very problematic species in Turkey in the comparison to other members of the genus. Distribution area for Q. calliprinos is east mediterranean region and seperated from Q. coccifera with different living area. These taxonomical problems indicate that the real phenetics and fylogenetics relations within Ilex section have not still been fully explained. Random amplıfıed polymorphıc DNA (RAPD) is a polymerase chain reaction (PCR) based technique used to show polymorphism among species This method is very helpful for systematics purposes and phylogenetic relation. According to the results of statistical analyses, it was attempted to draw the most possible borders of taxa based on the DNA bands obtained from RAPD analyses (Sesli and Yegenoglu, 2009; Açık et al, 2009; Kavalcıoglu et al, 2010) and a better phenetic classification by using molecular characters showing high correlations with each other
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