Abstract
Periodontitis is an inflammatory process that occurs in the tissues surrounding the teeth. It is caused by microorganisms and groups of microorganisms and results in loss of periodontal ligament and alveolar bone. Herpes virus has emerged as a putative pathogen in periodontal disease. Periodontal disease was demonstrated in 50 patients by careful probing of pockets and completion of a medical record. Three saliva samples were collected in individuals with deep pockets. Samples were obtained with a sterile swab and placed in 10% glucose solution for storage at -80°C. DNA purification and PCR, in addition to electrophoresis, were performed. Of the 50 samples processed, two were positive for HSV-1 and two for HSV-2. Three were from women with mild, moderate, and severe chronic periodontitis, respectively, and one (HSV-1) from a man with mild chronic periodontitis. Due to the low prevalence of herpes simplex virus in periodontal pockets, it was not possible to determine its role in the progression of periodontitis. Key words: Herpes simplex virus, plaque index, gingival index, electrophoresis.
Highlights
Periodontitis is an inflammatory process that occurs in the tissues surrounding the teeth
We studied 50 patients with chronic periodontitis; 32 women and 18 men with a mean age of 41 years and a range of 24 to 70 years
Mild (4) and moderate (3) periodontitis were predominant in women 20-35 years; mild (2) and moderate (12) disease with a few advanced cases (2) were predominant in those 36-50 years old; and in those >51 years, mild (3) and moderate (2) periodontitis were predominant with several advanced cases (3)
Summary
Periodontitis is an inflammatory process that occurs in the tissues surrounding the teeth It is caused by microorganisms and groups of microorganisms and results in progressive destruction of the periodontal ligament and alveolar bone (Loesche and Grossman, 2001). It is important to consider the composition of the subgingival microflora since this varies significantly when the periodontium is pathologically affected. Previous research on this disorder has focused on putative periodontal bacteria and their products (Saygun et al, 2004)
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