Abstract
Basal stem rot (BSR) disease caused by the fungus, Ganoderma boninense has become a serious threat to the oil palm industry, especially in Southeast Asia (SEA). A highly selective and sensitive diagnostic tool for BSR is extremely required for early detection, and thus, development of immunological test using enzyme-linked immunosorbent assay-polyclonal antibody (ELISA-PAb) was evaluated. Results indicate that ELISA-PAb shows recognition of Ganoderma species associated with BSR except for G. tornatum. Cross-reactivity test with fungi commonly found in oil palm plantation revealed observation of some cross-reactions with some saprophytic fungi. ELISA-PAb shows better detection as compared to cultural-based method, Ganoderma selective medium (GSM) with an improvement of 18% at nursery trial. The present study also demonstrates sensitive detection on ELISA-PAb with an increment of 30% as compared to GSM test at field trial using oil palm roots and stems. Polyclonal antibodies raised against G. boninense with positive signals was achieved, however, not specific enough for detection of BSR disease caused by Ganoderma. Key words: Ganoderma boninense, basal stem rot, polyclonal antibodies, enzyme-linked immunosorbent assay (ELISA).
Highlights
Polyclona al antib bodies s of Ga anoderrma bo oninens se isolated from m Malay ysian oil o palm m for d detection of basal stem rot r disease
We describe the production and application of specific PAbs against G. boninense for basal stem rot (BSR) disease detection using modified enzyme-linked immunosorbent assay (ELISA) method
This article provides an overview of polyclonal antibody approach and its application in detection of Ganoderma disease is one of decision-making tool for an early detection in nursery and field
Summary
Polyclona al antib bodies s of Ga anoderrma bo oninens se isolated from m Malay ysian oil o palm m for d detection of basal stem rot r disease. Basal stem rot (BSR) diisease cause ed by the fun ngus, Ganod derma bonine ense has bec come a serio ous threat to the oil pa alm industry y, especially in Southeas st Asia (SEA)). Ab shows rec cognition of Ganoderma a species as ssociated witth BSR exce ept for G. tornatum. Cross-reactiv vity test with fungi comm monly found in oil palm p plantation rev vealed obserrvation of some cross s-reactions with w some sa aprophytic fu ungi. ELISA--PAb shows better detec ction as com mpared to cultural-bas sed method,, Ganoderma a selective medium m
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