Abstract
Ganoderma sinensis immunomodulatory protein (FIP-gsi) was a new protein in fungal immunomodulatory protein (FIP) family. Based on the recombinant FIP-gsi expressed inEscherichia coli, the New Zealand white rabbits were immunized with the purity protein to prepare anti-FIP-gsi polyclonal antibody. The efficacy of polyclonal antibody was detected by ELISA and Western blot. The results showed that the anti-FIP-gsi polyclonal antibody with high efficient value and specificity has been successfully preparation, and its efficient value was 1:625,000 detected by indirect ELISA, and a special band had been observed by Western blot method. This study established a method to identify FIP-gsi by immunoblotting, and will lay a foundation for further exploring the immunologic function of FIP-gsi. Key words: Ganoderma sinensis fugal immunomodulatory proteins (FIP-gsi), polyclonal antibody, ELISA, Western blot.
Highlights
The first fungal immunomodulatory protein (FIP), LZ-8, was isolated from Ganoderma lucidum in 1989 (Kino et al, 1989)
Based on the recombinant FIP-gsi expressed in Escherichia coli, the New Zealand white rabbits were immunized with the purity protein to prepare anti-FIP-gsi polyclonal antibody
The efficacy of polyclonal antibody was detected by ELISA and Western blot
Summary
The first fungal immunomodulatory protein (FIP), LZ-8, was isolated from Ganoderma lucidum in 1989 (Kino et al, 1989). Based on the recombinant FIP-gsi expressed in Escherichia coli, the New Zealand white rabbits were immunized with the purity protein to prepare anti-FIP-gsi polyclonal antibody. The efficacy of polyclonal antibody was detected by ELISA and Western blot. The results showed that the anti-FIP-gsi polyclonal antibody with high efficient value and specificity has been successfully preparation, and its efficient value was 1:625,000 detected by indirect ELISA, and a special band had been observed by Western blot method.
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