Abstract
Wheat (Triticum aestivum L.) is one of the most important food crops worldwide. Powdery mildew (Pm), caused by Blumeria graminis f. sp. tritici is a severe disease in wheat production. Gene Pm5e, from a Chinese wheat cultivar Fuzhuang 30 has proven to be a valuable resistance source for Pm in breeding. To further map this gene and develop Kompetitive allele-specific PCR (KASP) assays for marker-assisted selection (MAS), a F2 population containing 395 individuals was first phenotyped for Pm resistance, a bulked segregant analysis (BSA) was used to identify polymorphic SNPs using the 35K wheat SNPs chip. 27 polymorphic SNPs between bulks in the Pm5e region were identified and were converted into KASP assays to map Pm5e. A genetic linkage map of Pm5e was constructed with 2 SNP and 2 SSR molecular markers. Pm5e was mapped to a 9.5 cM interval and the two SNP markers AX-95000860 and AX-94638908 were the two closet flanking markers, which delimited Pm5e into a 14 Mb region. Identification of the molecular markers and development of the two KASP assays laid a solid base for MAS of gene Pm5e in breeding. Key words: Linkage map, marker assisted selection, SNP marker, wheat
Highlights
Wheat is an adaptable and widely distributed world food crop, which provide about 21% of food calories and 20% of protein for the human (He et al, 2018)
Powdery mildew (Pm) is a disease caused by the biotrophic fungus Blumeria graminis f. sp. tritici (Bgt), which often occurs in wheat production areas with cool and humid climates (Cowger et al, 2012)
The objective of this study is to: (1) identify single nucleotide polymorphism (SNP) markers closely linked with Pm5e, and (2) develop Kompetitive allele-specific PCR (KASP) assays that can be widely used in markerassisted selection (MAS) of Pm5e to improve Pm resistance in wheat
Summary
Wheat is an adaptable and widely distributed world food crop, which provide about 21% of food calories and 20% of protein for the human (He et al, 2018). Tritici (Bgt), which often occurs in wheat production areas with cool and humid climates (Cowger et al, 2012). In China, this foliar disease is endangering most regions of winter wheat and spring wheat productions (Liu et al, 2016). Seventy-eight designated and many other temporarily designated Pm resistance genes or alleles have been identified in wheat. Some of these genes have single alleles, while some of them have multiple alleles (e.g., Pm1, Pm2, Pm3, Pm4, Pm5, and Pm54 loci) (Wu et al, 2018; Zhang et al, 2016)
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