Abstract
Nine samples of goat’s cheese were collected. A total of 68 yeasts were isolated and grouped according to their M13 PCR-fingerprints. Representative isolates of each fingerprint group were identified using rRNA and/or protein-coding gene sequencing leading to the identification of 18 yeast species. The dominant species were Kluyveromyces lactis (19.1%), Saccharomyces cerevisiae (11.7%), Yarrowia lipolytica (10.3%), Candida parapsilosis (10.3%), Kazachstania unispora (8.0%), Kluyveromyces marxianus (7.4%) and Pichia fermentans (5.9%). The yeast diversity of Moroccan goat’s cheese was established using genotypic techniques which proved to be a straightforward approach for the identii¬cation of all isolates. This work yielded a well-characterized collection of yeasts from traditional and semi-industrial Moroccan goat cheeses which will be a resource of strains with specii¬c properties. Key words: Yeasts, goat cheese, identification, genotypic approach.
Highlights
Mycothèque de d l'Université é catholique de d Louvain, Belgian Coord inated Collecction of Microo organisms, Ea arth and Life
Thirty four isolates were correctly identified by API ID32C, while 14 isolates were incorrectly identified and 20 isolates could not be identified by API ID32C
Isolates identified as K. lactis and K. marxianus, but none of the other species were able to utilize lactose as a carbon source (Table 2)
Summary
Mycothèque de d l'Université é catholique de d Louvain, Belgian Coord inated Collecction of Microo organisms, Ea arth and Life. Nine samples of goat’s cheese were e collected. A total of 68 yeasts were e isolated an nd grouped a according to their M13 PCR-finge erprints. Rep presentative isolates of e each fingerp print group w were identifiied using rRNA and/o or protein-co oding gene sequencing g leading to the identific cation of 18. The yeas st diversity of Moroccan n goat’s che eese was established d using gen notypic tech hniques which proved to be a sttraightforwarrd approach h for the identificatio on of all isolates. Goat’s milk k cheeses are produced in many Mediterran nean countrie es such as Spain. B of goa at cheese on n sale vary fro om one e locality to another but all supermarrkets offer go oat che eeses
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