Abstract
Midkine (MK) is a heparin-binding growth factor and was found to be highly expressed in many types of human carcinomas. MK may become a novel tumor marker. In this study, we used the rabbit specific antibodies against human MK to establish a double antibody sandwich enzyme-linked immunosorbent assay (ELISA) of MK system and applied it to detect serum MK levels in different types of cancer patients. The standard curve, precision and recovery rate were tested, respectively, and serum MK concentration of 102 cancers patients and 102 normal individuals were detected using this method. The detection range of this method was 0.2 to 10 ng/ml (R2 = 0.97). The average intra and intro-assay coefficients of variation (CVs) were 3.6 and 7.9%, respectively. The average recovery rate was 89.9% when some standard antigens were added into the serum. The medians (25th and 75th percentiles) of serum MK levels were 1.35 ng/ml (0.96 and 1.64) in cancer patients and 0.30 ng/ml (0.23 and 0.38) in the controls; the MK levels of the patients were significantly higher than those of the controls (P<0.05). Moreover, 87.2% of the patients showed more than 0.6 ng/ml levels of MK. Serum MK could serve as a general tumor marker with a good potential for clinical application. Key words: Midkine, rabbit monoclonal antibody, sandwich ELISA, tumor marker.
Highlights
Midkine (MK) is a heparin-binding growth factor found as the product of a retinoic acid-responsive gene (Kadomatsu et al, 1988)
It has 45% sequence identity to pleiotrophin [(PTN)/heparin-binding growth-associated molecule (HB-GAM)], and together, these molecules comprise a family of heparin-binding growth factors (Tomomura et al, 1990)
In this enzyme-linked immunosorbent assay (ELISA), dose-dependent increase in the absorbance at 450 nm enables the measuring of MK ranging between 0.2 and 10 ng/ml
Summary
Midkine (MK) is a heparin-binding growth factor found as the product of a retinoic acid-responsive gene (Kadomatsu et al, 1988). We used the rabbit specific antibodies against human MK to establish a double antibody sandwich enzyme-linked immunosorbent assay (ELISA) of MK system and applied it to detect serum MK levels in different types of cancer patients. The standard curve, precision and recovery rate were tested, respectively, and serum MK concentration of 102 cancers patients and 102 normal individuals were detected using this method.
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
